The international normalized ratio according to Owren in liver disease: interlaboratory assessment and determination of international sensitivity index.
Thromb Res
; 132(3): 346-51, 2013 Sep.
Article
em En
| MEDLINE
| ID: mdl-23993902
ABSTRACT
INTRODUCTION:
The international normalized ratio (INR) is used to prioritize liver disease patients for transplantation. Previous studies have shown high interlaboratory variability in Quick-based INR determinations in samples of patients with liver disease. We assessed Owren-based INR reagents for analyzing INR in patients with liver disease. Further, we determined the difference between international sensitivity index (ISI) for patients on vitamin K antagonists (ISIVKA) and ISI for patients with liver disease (ISIliver). PATIENTS ANDMETHODS:
Twenty patients with liver disease were included, 10 with INR 1.8-3.6 (group A1) and 10 with INR 1.2-1.5 (group C1). Plasma from these patients was analyzed for Owren-based INR in eight Swedish laboratories using either of following reagents SPA+, Owrens PT or Nycotest PT. To determine ISI liver, the reference thromboplastin RBT/05 and additional 41 patients with liver disease and 20 normal controls were included. ISIVKA was determined according to the WHO procedure. The difference between the ISIVKA and ISIliver was calculated.RESULTS:
The coefficients of variance for the Owren based INR methods were 6.2% in group A1, 3.9 % in group C1 and 5.3% for all patients. The difference between ISIVKA and ISIliver were -0.4%, -0.7% and -0.2% for SPA+, Owrens PT and Nycotest PT respectively.CONCLUSIONS:
Interlaboratory variation in INR analyses according to Owren in patients with liver disease is low and the difference between ISIVKA and ISIliver is below 10% with this method. ISIVKA can therefore be used in the INR calibration, for the Owren reagents studied, when analyzing plasma from patients with liver disease.Palavras-chave
CV; EQUALIS; External Quality Assurance of Laboratory medicine; FII; FV; FVII; FX; INR; ISI; ISI(VKA); ISI(liver); Liver disease; Liver transplantation; MELD; PELD; PPP; PT; Prothrombin time; SSC; Severity of illness index; VKA; coefficients of variation; factor II; factor V; factor VII; factor X; international normalized ratio; international sensitivity index; international sensitivity index for patients on vitamin K antagonists; international sensitivity index for patients with liver disease; model of end-stage liver disease; pediatric end-stage liver disease; platelet poor plasma; prothrombin time; the Scientific and Standardisation Committee of the International Society on Thrombosis and Haemostasis; vitamin K antagonists
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Transplante de Fígado
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Coeficiente Internacional Normatizado
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Hepatopatias
Tipo de estudo:
Diagnostic_studies
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Prognostic_studies
Limite:
Adolescent
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Adult
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Aged
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Aged80
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Female
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Humans
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Male
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Middle aged
Idioma:
En
Revista:
Thromb Res
Ano de publicação:
2013
Tipo de documento:
Article