Profiling the triacylglyceride contents in bat integumentary lipids by preparative thin layer chromatography and MALDI-TOF mass spectrometry.
J Vis Exp
; (79)2013 Sep 05.
Article
em En
| MEDLINE
| ID: mdl-24056580
ABSTRACT
The mammalian integument includes sebaceous glands that secrete an oily material onto the skin surface. Sebum production is part of the innate immune system that is protective against pathogenic microbes. Abnormal sebum production and chemical composition are also a clinical symptom of specific skin diseases. Sebum contains a complex mixture of lipids, including triacylglycerides, which is species-specific. The broad chemical properties exhibited by diverse lipid classes hinder the specific determination of sebum composition. Analytical techniques for lipids typically require chemical derivatizations that are labor-intensive and increase sample preparation costs. This paper describes how to extract lipids from mammalian integument, separate broad lipid classes by thin-layer chromatography, and profile the triacylglyceride contents using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. This robust method enables a direct determination of the triacylglyceride profiles among species and individuals, and it can be readily applied to any taxonomic group of mammals.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Triglicerídeos
/
Asas de Animais
/
Quirópteros
/
Cromatografia em Camada Fina
/
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
/
Tegumento Comum
/
Lipídeos
Limite:
Animals
Idioma:
En
Revista:
J Vis Exp
Ano de publicação:
2013
Tipo de documento:
Article