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Thymoquinone prevents ß-amyloid neurotoxicity in primary cultured cerebellar granule neurons.
Ismail, Norsharina; Ismail, Maznah; Mazlan, Musalmah; Latiff, Latiffah Abdul; Imam, Mustapha Umar; Iqbal, Shahid; Azmi, Nur Hanisah; Ghafar, Siti Aisyah Abd; Chan, Kim Wei.
Afiliação
  • Ismail N; Nutricosmeceuticals and Nutrigenomics Programme, Laboratory of Molecular Biomedicine, Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia.
Cell Mol Neurobiol ; 33(8): 1159-69, 2013 Nov.
Article em En | MEDLINE | ID: mdl-24101432
Thymoquinone (TQ), a bioactive constituent of Nigella sativa Linn (N. sativa) has demonstrated several neuropharmacological attributes. In the present study, the neuroprotective properties of TQ were investigated by studying its anti-apoptotic potential to diminish ß-amyloid peptide 1-40 sequence (Aß1-40)-induced neuronal cell death in primary cultured cerebellar granule neurons (CGNs). The effects of TQ against Aß1-40-induced neurotoxicity, morphological damages, DNA condensation, the generation of reactive oxygen species, and caspase-3, -8, and -9 activation were investigated. Pretreatment of CGNs with TQ (0.1 and 1 µM) and subsequent exposure to 10 µM Aß1-40 protected the CGNs against the neurotoxic effects of the latter. In addition, the CGNs were better preserved with intact cell bodies, extensive neurite networks, a loss of condensed chromatin and less free radical generation than those exposed to Aß1-40 alone. TQ pretreatment inhibited Aß1-40-induced apoptosis of CGNs via both extrinsic and intrinsic caspase pathways. Thus, the findings of this study suggest that TQ may prevent neurotoxicity and Aß1-40-induced apoptosis. TQ is, therefore, worth studying further for its potential to reduce the risks of developing Alzheimer's disease.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Cerebelo / Peptídeos beta-Amiloides / Benzoquinonas / Fármacos Neuroprotetores / Neurônios / Neurotoxinas Limite: Animals Idioma: En Revista: Cell Mol Neurobiol Ano de publicação: 2013 Tipo de documento: Article País de afiliação: Malásia País de publicação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Cerebelo / Peptídeos beta-Amiloides / Benzoquinonas / Fármacos Neuroprotetores / Neurônios / Neurotoxinas Limite: Animals Idioma: En Revista: Cell Mol Neurobiol Ano de publicação: 2013 Tipo de documento: Article País de afiliação: Malásia País de publicação: Estados Unidos