Fully-automated radiosynthesis and in vitro uptake investigation of [N-methyl-¹¹C]methylene blue.
Anticancer Res
; 33(10): 4267-70, 2013 Oct.
Article
em En
| MEDLINE
| ID: mdl-24122991
ABSTRACT
Malignant melanoma is a type of skin cancer which can spread rapidly if not detected early and left untreated. Positron Emission Tomography (PET) is a powerful imaging technique for detecting cancer but with only a limited number of radiotracers available the development of novel PET probes for detection and prevention of cancer is imperative. In the present study we present the fully-automated radiosynthesis of [N-methyl-(11)C]methylene blue and an in vitro uptake study in metastasic melanoma cell lines. Using the GE TRACERlab FXc Pro module [N-methyl-(11)C]methylene blue was isolated via solid-phase extraction in an average time of 36 min after end of bombardment and formulated with a radiochemical purity greater than 95%. The in vitro uptake study of [N-methyl-(11)C]methylene blue in SK-MEL28 melanin-expressing melanoma cell line demonstrated in site-specific binding of 51% promoting it as a promising melanoma PET imaging agent.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Compostos Radiofarmacêuticos
/
Azul de Metileno
Limite:
Humans
Idioma:
En
Revista:
Anticancer Res
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Reino Unido