Gating charge movement precedes ionic current activation in hERG channels.
Channels (Austin)
; 8(1): 84-9, 2014.
Article
em En
| MEDLINE
| ID: mdl-24126078
ABSTRACT
We recently reported gating currents recorded from hERG channels expressed in mammalian TSA cells and assessed the kinetics at different voltages. We detected 2 distinct components of charge movement with the bulk of the charge being carried by a slower component. Here we compare our findings in TSA cells with recordings made from oocytes using the Cut Open Vaseline Gap clamp (COVG) and go on to directly compare activation of gating charge and ionic currents at 0 and +60 mV. The data show that gating charge saturates and moves more rapidly than ionic current activates suggesting a transition downstream from the movement of the bulk of gating charge is rate limiting for channel opening.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ativação do Canal Iônico
/
Canais de Potássio Éter-A-Go-Go
Limite:
Humans
Idioma:
En
Revista:
Channels (Austin)
Ano de publicação:
2014
Tipo de documento:
Article