The need for transparency and good practices in the qPCR literature.

Bustin, Stephen A; Benes, Vladimir; Garson, Jeremy; Hellemans, Jan; Huggett, Jim; Kubista, Mikael; Mueller, Reinhold; Nolan, Tania; Pfaffl, Michael W; Shipley, Gregory; Wittwer, Carl T; Schjerling, Peter; Day, Philip J; Abreu, Mónica; Aguado, Begoña; Beaulieu, Jean-François; Beckers, Anneleen; Bogaert, Sara; Browne, John A; Carrasco-Ramiro, Fernando; Ceelen, Liesbeth; Ciborowski, Kate; Cornillie, Pieter; Coulon, Stephanie; Cuypers, Ann; De Brouwer, Sara; De Ceuninck, Leentje; De Craene, Jurgen; De Naeyer, Hélène; De Spiegelaere, Ward; Deckers, Kato; Dheedene, Annelies; Durinck, Kaat; Ferreira-Teixeira, Margarida; Fieuw, Annelies; Gallup, Jack M; Gonzalo-Flores, Sandra; Goossens, Karen; Heindryckx, Femke; Herring, Elizabeth; Hoenicka, Hans; Icardi, Laura; Jaggi, Rolf; Javad, Farzad; Karampelias, Michael; Kibenge, Frederick; Kibenge, Molly; Kumps, Candy; Lambertz, Irina; Lammens, Tim

Bustin, Stephen A; Benes, Vladimir; Garson, Jeremy; Hellemans, Jan; Huggett, Jim; Kubista, Mikael; Mueller, Reinhold; Nolan, Tania; Pfaffl, Michael W; Shipley, Gregory; Wittwer, Carl T; Schjerling, Peter; Day, Philip J; Abreu, Mónica; Aguado, Begoña; Beaulieu, Jean-François; Beckers, Anneleen; Bogaert, Sara; Browne, John A; Carrasco-Ramiro, Fernando; Ceelen, Liesbeth; Ciborowski, Kate; Cornillie, Pieter; Coulon, Stephanie; Cuypers, Ann; De Brouwer, Sara; De Ceuninck, Leentje; De Craene, Jurgen; De Naeyer, Hélène; De Spiegelaere, Ward; Deckers, Kato; Dheedene, Annelies; Durinck, Kaat; Ferreira-Teixeira, Margarida; Fieuw, Annelies; Gallup, Jack M; Gonzalo-Flores, Sandra; Goossens, Karen; Heindryckx, Femke; Herring, Elizabeth; Hoenicka, Hans; Icardi, Laura; Jaggi, Rolf; Javad, Farzad; Karampelias, Michael; Kibenge, Frederick; Kibenge, Molly; Kumps, Candy; Lambertz, Irina; Lammens, Tim.

Afiliação

Bustin SA; Postgraduate Medical Institute, Anglia Ruskin University, Chelmsford, UK.

Nat Methods ; 10(11): 1063-7, 2013 Nov.

Article em En | MEDLINE | ID: mdl-24173381

ABSTRACT

ABSTRACT

Two surveys of over 1,700 publications whose authors use quantitative real-time PCR (qPCR) reveal a lack of transparent and comprehensive reporting of essential technical information. Reporting standards are significantly improved in publications that cite the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines, although such publications are still vastly outnumbered by those that do not.

Assuntos

Serviços de Informação; Reação em Cadeia da Polimerase/métodos; Coleta de Dados

Texto completo

Adicionar na Minha BVS

Imprimir

XML

PubMed Links

Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Reação em Cadeia da Polimerase / Serviços de Informação Tipo de estudo: Qualitative_research Idioma: En Revista: Nat Methods Assunto da revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Ano de publicação: 2013 Tipo de documento: Article País de afiliação: Reino Unido

Texto completo

Adicionar na Minha BVS

Imprimir

XML

PubMed Links

Buscar no Google