Agrobacterium-mediated transformation of white mustard (Sinapis alba L.) and regeneration of transgenic plants.
Plant Cell Rep
; 13(3-4): 130-4, 1994 Jan.
Article
em En
| MEDLINE
| ID: mdl-24193637
ABSTRACT
A procedure for the regeneration of fertile transgenic white mustard (Sinapis alba L.) is presented. The protocol is based on infection of stem explants of 7-9 day old plants with an Agrobacterium tumefaciens strain harboring a disarmed binary vector with chimeric genes encoding neomycin phosphotransferase and ß-glucuronidase. Shoots are regenerated from callus-forming explants within 3-4 weeks. Under selection, 10% of the explants with transgenic embryonic callus develop into fertile transgenic plants. Rooting shoots transferred to soil yield seeds within 14-16 weeks following transformation. Integration and expression of the T-DNA encoded marker genes was confirmed by histochemical ß glucuronidase assays and Southern-DNA hybridization using primary transformants and S1-progeny. The analysis showed stable integration and Mendelian inheritance of trans-genes in transformed Sinapis lines.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
Plant Cell Rep
Assunto da revista:
BOTANICA
Ano de publicação:
1994
Tipo de documento:
Article
País de afiliação:
Alemanha