Development of ionic channels during mouse neuronal differentiation.
J Physiol (Paris)
; 80(4): 312-20, 1985.
Article
em En
| MEDLINE
| ID: mdl-2422359
ABSTRACT
Using a mouse embryonal teratocarcinoma (E.C.) cell line, it was possible to follow the sequence of development of ionic channels during neuronal differentiation, with patch-clamp techniques. 1003 E.C. cells were induced to differentiate into neurons by culturing them in defined medium without foetal calf serum (DARMON et al., 1981). Non-differentiated cells were not excitable and presented mainly 2 types of K+ channels a Ca2+ activated K+ channel (220 pS in symmetrical K+) and a delayed rectifier (30 pS in symmetrical K+). When the cells start to grow neurites, a low threshold calcium current can be recorded, only if the cell is held at hyperpolarized potentials (-70 to -80 mV). Fully differentiated cells with long neurites presented a complete repertoire of ionic channels voltage dependent Na+ and Ca2+ channels, Ca2+ activated K+ channel and K+ delayed rectifier.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Canais Iônicos
/
Neurônios
Limite:
Animals
Idioma:
En
Revista:
J Physiol (Paris)
Assunto da revista:
FISIOLOGIA
Ano de publicação:
1985
Tipo de documento:
Article