An enzyme-linked immunosorbent assay for detection of avian influenza virus subtypes H5 and H7 antibodies.
Acta Vet Scand
; 55: 84, 2013 Nov 21.
Article
em En
| MEDLINE
| ID: mdl-24256721
ABSTRACT
BACKGROUND:
Avian influenza virus (AIV) subtypes H5 and H7 attracts particular attention because of the risk of their potential pathogenicity in poultry. The haemagglutination inhibition (HI) test is widely used as subtype specific test for serological diagnostics despite the laborious nature of this method. However, enzyme-linked immunosorbent assays (ELISAs) are being explored as an alternative test method.H5 and H7 specific monoclonal antibodies were experimentally raised and used in the development of inhibition ELISAs for detection of serological response specifically directed against AIV subtypes H5 and H7. The ELISAs were evaluated with polyclonal chicken anti-AIV antibodies against AIV subtypes H1N2, H5N2, H5N7, H7N1, H7N7, H9N9, H10N4 and H16N3.RESULTS:
Both the H5 and H7 ELISA proved to have a high sensitivity and specificity and the ELISAs detected H5 and H7 antibodies earlier during experimental infection than the HI test did. The reproducibility of the ELISA's performed at different times was high with Pearson correlation coefficients of 0.96-0.98.CONCLUSIONS:
The ELISAs are a potential alternative to the HI test for screening of large amounts of avian sera, although only experimental sera were tested in this study.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vírus da Influenza A
/
Ensaio de Imunoadsorção Enzimática
/
Glicoproteínas de Hemaglutininação de Vírus da Influenza
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Influenza Aviária
/
Anticorpos Antivirais
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Revista:
Acta Vet Scand
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Dinamarca
País de publicação:
ENGLAND
/
ESCOCIA
/
GB
/
GREAT BRITAIN
/
INGLATERRA
/
REINO UNIDO
/
SCOTLAND
/
UK
/
UNITED KINGDOM