Upregulation of GM-CSF by TGF-ß1 in epithelial mesenchymal transition of human HERS/ERM cells.
In Vitro Cell Dev Biol Anim
; 50(5): 399-405, 2014.
Article
em En
| MEDLINE
| ID: mdl-24258001
ABSTRACT
Hertwig's epithelial root sheath/epithelial rests of Malassez (HERS/ERM) have been suggested to play an important role in tooth root formation, particularly in periodontal development. Epithelial mesenchymal transition (EMT) has been suggested to contribute to root development in tooth. However, the mechanism of interaction between HERS/ERM cells and dental mesenchymal cells has not been fully understood. In this study, we investigated the effect of exogenous transforming growth factor beta 1 (TGF-ß1) in human HERS/ERM cells in order to verify the role of granulocyte macrophage colony-stimulating factor (GM-CSF) in EMT process. Antibody array was used to screen secretion factors by exogenous TGF-ß1. Secretion of GM-CSF was increased by exogenous TGF-ß1. Expression levels of EMT markers, vimentin, ZEB1 (zinc finger E-box binding homeobox 1), and E-cadherin, were confirmed using reverse transcription polymerase chain reaction and immunocytochemistry. Treatment with GM-CSF increased the expression of vimentin and ZEB1, similar to TGF-ß1 treatment, and decreased the expression of E-cadherin. Our results suggest that GM-CSF could induce EMT in human HERS/ERM cells.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Raiz Dentária
/
Fator Estimulador de Colônias de Granulócitos e Macrófagos
/
Fator de Crescimento Transformador beta1
/
Transição Epitelial-Mesenquimal
Limite:
Humans
Idioma:
En
Revista:
In Vitro Cell Dev Biol Anim
Assunto da revista:
BIOLOGIA
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
Coréia do Sul