High-performance liquid chromatography with fluorescence detection for the determination of nitrofuran metabolites in pork muscle.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess
; 30(12): 2114-22, 2013.
Article
em En
| MEDLINE
| ID: mdl-24283965
ABSTRACT
A simple and sensitive HPLC method with fluorescence detection (HPLC-FLD) is reported for the simultaneous determination of metabolites of four nitrofuran drugs (furazolidone, furaltadone, nitrofurantoin and nitrofurazone) in pork muscle. The method involves acid hydrolysis of the protein-bound drug metabolites and the conjugation of the released side-chains with a novel fluorescence agent 2-hydroxy-1-naphthaldehyde. After liquid-liquid extraction and effective separation of the derivatives on a YMC-Pack Polymer C18 column at 40°C under alkaline conditions, the high fluorescence intensity of these derivatives at emission wavelength λem = 463 nm enables their simultaneous determination in pork muscle at concentrations as low as 1 µg kg⻹. The method was validated using blank pork muscle fortified with all four metabolites at 0.5, 1.0 and 2.0 µg kg⻹. Recoveries were > 92.3% with RSDs < 8.5% for all four metabolites. The results obtained with HPLC-FLD and LC-MS/MS methods showed very good agreement for pork muscle samples.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Resíduos de Drogas
/
Carcinógenos
/
Contaminação de Alimentos
/
Inspeção de Alimentos
/
Carne
/
Antibacterianos
/
Nitrofuranos
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
País/Região como assunto:
Asia
Idioma:
En
Revista:
Food Addit Contam Part A Chem Anal Control Expo Risk Assess
Assunto da revista:
CIENCIAS DA NUTRICAO
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
China