Heterogeneity of glycolytic oscillatory behaviour in individual yeast cells.
FEBS Lett
; 588(1): 3-7, 2014 Jan 03.
Article
em En
| MEDLINE
| ID: mdl-24291821
ABSTRACT
There are many examples of oscillations in biological systems and one of the most investigated is glycolytic oscillations in yeast. These oscillations have been studied since the 1950s in dense, synchronized populations and in cell-free extracts, but it has for long been unknown whether a high cell density is a requirement for oscillations to be induced, or if individual cells can oscillate also in isolation without synchronization. Here we present an experimental method and a detailed kinetic model for studying glycolytic oscillations in individual, isolated yeast cells and compare them to previously reported studies of single-cell oscillations. The importance of single-cell studies of this phenomenon and relevant future research questions are also discussed.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Leveduras
/
Glicólise
/
Modelos Biológicos
/
NAD
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
2014
Tipo de documento:
Article