Hyperspectral darkfield microscopy of PEGylated gold nanoparticles targeting CD44-expressing cancer cells.
J Biophotonics
; 8(1-2): 162-7, 2015 Jan.
Article
em En
| MEDLINE
| ID: mdl-24343875
ABSTRACT
We present a new hyperspectral darkfield imaging system with a scanned broadband supercontinuum light source. We observed the specific attachment of the functionalized gold plasmonic nanoparticles (AuNPs) targeting CD44(+) human breast cancer cells by conventional and by proposed hyperspectral darkfield microscopy. This wide-field and low phototoxic hyperspectral imaging system has been successful for performing spectral three-dimensional (3D) localization and spectroscopic identification of CD44-targeted PEGylated AuNPs in fixed cell preparations. Such spatial and spectral information is essential for the improvement of nanoplasmonic-based imaging, disease detection and treatment in complex biological environment. Presented system capability for 3D NP tracking will also enable investigation of specific sub-cellular activity with the use of NPs as spectral sensors.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Polietilenoglicóis
/
Regulação Neoplásica da Expressão Gênica
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Receptores de Hialuronatos
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Nanopartículas Metálicas
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Ouro
/
Microscopia
Limite:
Humans
Idioma:
En
Revista:
J Biophotonics
Assunto da revista:
BIOFISICA
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
Canadá