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A plate-based electrochromic approach for the high-throughput detection of electrochemically active bacteria.
Yuan, Shi-Jie; Li, Wen-Wei; Cheng, Yuan-Yuan; He, Hui; Chen, Jie-Jie; Tong, Zhong-Hua; Lin, Zhi-Qi; Zhang, Feng; Sheng, Guo-Ping; Yu, Han-Qing.
Afiliação
  • Yuan SJ; 1] Department of Chemistry, University of Science and Technology of China, Hefei, China. [2].
  • Li WW; 1] Department of Chemistry, University of Science and Technology of China, Hefei, China. [2].
  • Cheng YY; Department of Chemistry, University of Science and Technology of China, Hefei, China.
  • He H; Department of Chemistry, University of Science and Technology of China, Hefei, China.
  • Chen JJ; Department of Chemistry, University of Science and Technology of China, Hefei, China.
  • Tong ZH; Department of Chemistry, University of Science and Technology of China, Hefei, China.
  • Lin ZQ; Department of Chemistry, University of Science and Technology of China, Hefei, China.
  • Zhang F; Department of Chemistry, University of Science and Technology of China, Hefei, China.
  • Sheng GP; Department of Chemistry, University of Science and Technology of China, Hefei, China.
  • Yu HQ; Department of Chemistry, University of Science and Technology of China, Hefei, China.
Nat Protoc ; 9(1): 112-9, 2014 Jan.
Article em En | MEDLINE | ID: mdl-24356770
Electrochemically active bacteria (EAB) have the ability to transfer electrons to electron acceptors located outside the cell, and they are widely present in diverse environments. In spite of their important roles in geochemical cycles, environmental remediation and electricity generation, so far, only a limited number and types of EAB have been isolated and characterized. Thus, effective and rapid EAB identification methods are highly desirable. In this protocol, we describe a photometric protocol for the visualization and high-throughput identification and isolation of EAB. The protocol relies on the fast electron acquisition and color change ability of an electrochromic material, namely a tungsten trioxide (WO3) nanorod assembly. The extracellular electron transfer (EET) from EAB to the WO3 nanorod assembly probe is accompanied by a bioelectrochromic reaction made evident by the color change of the probe. This protocol enables researchers to rapidly identify EAB and evaluate their EET ability either qualitatively with the naked eye or quantitatively by image analysis. We have also successfully used this protocol to isolate EAB from environmental samples. The time needed to complete this protocol is ∼2 d, with the actual EAB identification process taking about 5 min.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fotometria / Bactérias / Técnicas Eletroquímicas Tipo de estudo: Diagnostic_studies Idioma: En Revista: Nat Protoc Ano de publicação: 2014 Tipo de documento: Article País de publicação: Reino Unido

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fotometria / Bactérias / Técnicas Eletroquímicas Tipo de estudo: Diagnostic_studies Idioma: En Revista: Nat Protoc Ano de publicação: 2014 Tipo de documento: Article País de publicação: Reino Unido