Development of a multiplex PCR assay to detect Edwardsiella tarda, Streptococcus parauberis, and Streptococcus iniae in olive flounder (Paralichthys olivaceus).
J Vet Sci
; 15(1): 163-6, 2014.
Article
em En
| MEDLINE
| ID: mdl-24378589
ABSTRACT
A multiplex PCR protocol was established to simultaneously detect major bacterial pathogens in olive flounder (Paralichthys olivaceus) including Edwardsiella (E.) tarda, Streptococcus (S.) parauberis, and S. iniae. The PCR assay was able to detect 0.01 ng of E. tarda, 0.1 ng of S. parauberis, and 1 ng of S. iniae genomic DNA. Furthermore, this technique was found to have high specificity when tested with related bacterial species. This method represents a cheaper, faster, and reliable alternative for identifying major bacterial pathogens in olive flounder, the most important farmed fish in Korea.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Infecções Estreptocócicas
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Streptococcus
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Linguados
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Edwardsiella tarda
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Infecções por Enterobacteriaceae
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Reação em Cadeia da Polimerase Multiplex
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Doenças dos Peixes
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Pesqueiros
Tipo de estudo:
Diagnostic_studies
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Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
J Vet Sci
Assunto da revista:
MEDICINA VETERINARIA
Ano de publicação:
2014
Tipo de documento:
Article