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Use of osmium tetroxide staining with microcomputerized tomography to visualize and quantify bone marrow adipose tissue in vivo.
Scheller, Erica L; Troiano, Nancy; Vanhoutan, Joshua N; Bouxsein, Mary A; Fretz, Jackie A; Xi, Yougen; Nelson, Tracy; Katz, Griffin; Berry, Ryan; Church, Christopher D; Doucette, Casey R; Rodeheffer, Matthew S; Macdougald, Ormond A; Rosen, Clifford J; Horowitz, Mark C.
Afiliação
  • Scheller EL; Department of Molecular and Integrative Physiology, University of Michigan, Ann Arbor, Michigan, USA.
  • Troiano N; Department of Orthopaedics and Rehabilitation, Yale University School of Medicine, New Haven, Connecticut, USA.
  • Vanhoutan JN; Department of Internal Medicine, Endocrinology, Yale University School of Medicine, New Haven, Connecticut, USA.
  • Bouxsein MA; Center for Advanced Orthopedic Studies, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts, USA.
  • Fretz JA; Department of Orthopaedics and Rehabilitation, Yale University School of Medicine, New Haven, Connecticut, USA.
  • Xi Y; Department of Orthopaedics and Rehabilitation, Yale University School of Medicine, New Haven, Connecticut, USA.
  • Nelson T; Department of Orthopaedics and Rehabilitation, Yale University School of Medicine, New Haven, Connecticut, USA.
  • Katz G; Department of Orthopaedics and Rehabilitation, Yale University School of Medicine, New Haven, Connecticut, USA.
  • Berry R; Section of Comparative Medicine, Yale University School of Medicine, New Haven, Connecticut, USA.
  • Church CD; Section of Comparative Medicine, Yale University School of Medicine, New Haven, Connecticut, USA.
  • Doucette CR; Maine Medical Center Research Institute, Scarborough, Maine, USA.
  • Rodeheffer MS; Section of Comparative Medicine, Yale University School of Medicine, New Haven, Connecticut, USA.
  • Macdougald OA; Department of Molecular and Integrative Physiology, University of Michigan, Ann Arbor, Michigan, USA.
  • Rosen CJ; Maine Medical Center Research Institute, Scarborough, Maine, USA.
  • Horowitz MC; Department of Orthopaedics and Rehabilitation, Yale University School of Medicine, New Haven, Connecticut, USA. Electronic address: mark.horowitz@yale.edu.
Methods Enzymol ; 537: 123-39, 2014.
Article em En | MEDLINE | ID: mdl-24480344
Adipocytes reside in discrete, well-defined depots throughout the body. In addition to mature adipocytes, white adipose tissue depots are composed of many cell types, including macrophages, endothelial cells, fibroblasts, and stromal cells, which together are referred to as the stromal vascular fraction (SVF). The SVF also contains adipocyte progenitors that give rise to mature adipocytes in those depots. Marrow adipose tissue (MAT) or marrow fat has long been known to be present in bone marrow (BM) but its origin, development, and function remain largely unknown. Clinically, increased MAT is associated with age, metabolic diseases, drug treatment, and marrow recovery in children receiving radiation and chemotherapy. In contrast to the other depots, MAT is unevenly distributed in the BM of long bones. Conventional quantitation relies on sectioning of the bone to overcome issues with distribution but is time-consuming, resource intensive, inconsistent between laboratories and may be unreliable as it may miss changes in MAT volume. Thus, the inability to quantitate MAT in a rapid, systematic, and reproducible manner has hampered a full understanding of its development and function. In this chapter, we describe a new technique that couples histochemical staining of lipid using osmium tetroxide with microcomputerized tomography to visualize and quantitate MAT within the medullary canal in three dimensions. Imaging of osmium staining provides a high-resolution map of existing and developing MAT in the BM. Because this method is simple, reproducible, and quantitative, we expect it will become a useful tool for the precise characterization of MAT.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Tetróxido de Ósmio / Coloração e Rotulagem / Diferenciação Celular / Microtomografia por Raio-X Limite: Humans Idioma: En Revista: Methods Enzymol Ano de publicação: 2014 Tipo de documento: Article País de afiliação: Estados Unidos País de publicação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Tetróxido de Ósmio / Coloração e Rotulagem / Diferenciação Celular / Microtomografia por Raio-X Limite: Humans Idioma: En Revista: Methods Enzymol Ano de publicação: 2014 Tipo de documento: Article País de afiliação: Estados Unidos País de publicação: Estados Unidos