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Bio-imaging of hydroxyl radicals in plant cells using the fluorescent molecular probe rhodamine B hydrazide, without any pretreatment.
Asano, Mamiko; Doi, Mitsunobu; Baba, Kimiye; Taniguchi, Masahiko; Shibano, Makio; Tanaka, Satoshi; Sakaguchi, Minoru; Takaoka, Masanori; Hirata, Masahiko; Yanagihara, Rika; Nakahara, Ryosuke; Hayashi, Yuko; Yamaguchi, Takako; Matsumura, Hitoshi; Fujita, Yoshikazu.
Afiliação
  • Asano M; Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan. Electronic address: asanom@gly.oups.ac.jp.
  • Doi M; Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan.
  • Baba K; Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan.
  • Taniguchi M; Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan.
  • Shibano M; Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan.
  • Tanaka S; Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan.
  • Sakaguchi M; Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan.
  • Takaoka M; Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan.
  • Hirata M; Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan.
  • Yanagihara R; Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan.
  • Nakahara R; Department of Pharmacy, Oita University Hospital, 1-1 Idaigaoka Hasama-machi, Yuhu, Oita 879-5593, Japan.
  • Hayashi Y; Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan.
  • Yamaguchi T; Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan.
  • Matsumura H; Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan.
  • Fujita Y; Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan.
J Biosci Bioeng ; 118(1): 98-100, 2014 Jul.
Article em En | MEDLINE | ID: mdl-24485745
Rhodamine B hydrazide can be used to detect hydroxyl radicals in plant cells. RBH was easily inserted into plant cells without any pretreatment, and specifically reacted with intracellular hydroxyl radicals produced by antimycin A. RBH will be a powerful tool for detecting hydroxyl radicals in plant cells.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Rodaminas / Radical Hidroxila / Células Vegetais / Corantes Fluorescentes / Hidrazinas Idioma: En Revista: J Biosci Bioeng Assunto da revista: ENGENHARIA BIOMEDICA / MICROBIOLOGIA Ano de publicação: 2014 Tipo de documento: Article País de publicação: Japão
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Rodaminas / Radical Hidroxila / Células Vegetais / Corantes Fluorescentes / Hidrazinas Idioma: En Revista: J Biosci Bioeng Assunto da revista: ENGENHARIA BIOMEDICA / MICROBIOLOGIA Ano de publicação: 2014 Tipo de documento: Article País de publicação: Japão