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Kisspeptin modulates fertilization capacity of mouse spermatozoa.
Hsu, Meng-Chieh; Wang, Jyun-Yuan; Lee, Yue-Jia; Jong, De-Shien; Tsui, Kuan-Hao; Chiu, Chih-Hsien.
Afiliação
  • Hsu MC; Department of Animal Science and TechnologyCollege of Bio-Resources and Agriculture, National Taiwan University, No. 50, Ln. 155, Sec. 3, Keelung Road, Taipei 10673, TaiwanDepartment of Obstetrics and GynecologyKaohsiung Veterans General Hospital, Kaohsiung 81346, Taiwan, Republic of China.
  • Wang JY; Department of Animal Science and TechnologyCollege of Bio-Resources and Agriculture, National Taiwan University, No. 50, Ln. 155, Sec. 3, Keelung Road, Taipei 10673, TaiwanDepartment of Obstetrics and GynecologyKaohsiung Veterans General Hospital, Kaohsiung 81346, Taiwan, Republic of China.
  • Lee YJ; Department of Animal Science and TechnologyCollege of Bio-Resources and Agriculture, National Taiwan University, No. 50, Ln. 155, Sec. 3, Keelung Road, Taipei 10673, TaiwanDepartment of Obstetrics and GynecologyKaohsiung Veterans General Hospital, Kaohsiung 81346, Taiwan, Republic of China.
  • Jong DS; Department of Animal Science and TechnologyCollege of Bio-Resources and Agriculture, National Taiwan University, No. 50, Ln. 155, Sec. 3, Keelung Road, Taipei 10673, TaiwanDepartment of Obstetrics and GynecologyKaohsiung Veterans General Hospital, Kaohsiung 81346, Taiwan, Republic of China.
  • Tsui KH; Department of Animal Science and TechnologyCollege of Bio-Resources and Agriculture, National Taiwan University, No. 50, Ln. 155, Sec. 3, Keelung Road, Taipei 10673, TaiwanDepartment of Obstetrics and GynecologyKaohsiung Veterans General Hospital, Kaohsiung 81346, Taiwan, Republic of China.
  • Chiu CH; Department of Animal Science and TechnologyCollege of Bio-Resources and Agriculture, National Taiwan University, No. 50, Ln. 155, Sec. 3, Keelung Road, Taipei 10673, TaiwanDepartment of Obstetrics and GynecologyKaohsiung Veterans General Hospital, Kaohsiung 81346, Taiwan, Republic of China chiuchihh
Reproduction ; 147(6): 835-45, 2014 Jun.
Article em En | MEDLINE | ID: mdl-24567427
Kisspeptin acts as an upstream regulator of the hypothalamus-pituitary-gonad axis, which is one of the main regulatory systems for mammalian reproduction. Kiss1 and its receptor Kiss1r (also known as G protein-coupled receptor 54 (Gpr54)) are expressed in various organs, but their functions are not well understood. The purpose of this study was to investigate the expression profiles and functions of kisspeptin and KISS1R in the reproductive tissues of imprinting control region mice. To identify the expression pattern and location of kisspeptin and KISS1R in gonads, testes and ovarian tissues were examined by immunohistochemical or immunofluorescent staining. Kisspeptin and KISS1R were expressed primarily in Leydig cells and seminiferous tubules respectively. KISS1R was specifically localized in the acrosomal region of spermatids and mature spermatozoa. Kisspeptin, but not KISS1R, was expressed in the cumulus-oocyte complex and oviductal epithelium of ovarian and oviductal tissues. The sperm intracellular calcium concentrations significantly increased in response to treatment with kisspeptin 10 in Fluo-4-loaded sperm. The IVF rates decreased after treatment of sperm with the kisspeptin antagonist peptide 234. These results suggest that kisspeptin and KISS1R might be involved in the fertilization process in the female reproductive tract. In summary, this study indicates that kisspeptin and KISS1R are expressed in female and male gametes, respectively, and in mouse reproductive tissues. These data strongly suggest that the kisspeptin system could regulate mammalian fertilization and reproduction.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Espermatozoides / Fertilização / Kisspeptinas Tipo de estudo: Prognostic_studies Limite: Animals / Pregnancy Idioma: En Revista: Reproduction Assunto da revista: MEDICINA REPRODUTIVA Ano de publicação: 2014 Tipo de documento: Article País de afiliação: China País de publicação: Reino Unido

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Espermatozoides / Fertilização / Kisspeptinas Tipo de estudo: Prognostic_studies Limite: Animals / Pregnancy Idioma: En Revista: Reproduction Assunto da revista: MEDICINA REPRODUTIVA Ano de publicação: 2014 Tipo de documento: Article País de afiliação: China País de publicação: Reino Unido