In vitro transfection of bone marrow-derived dendritic cells with TATp-liposomes.
Int J Nanomedicine
; 9: 963-73, 2014.
Article
em En
| MEDLINE
| ID: mdl-24611012
ABSTRACT
Dendritic cells (DC) are antigen-presenting cells uniquely capable of priming naïve T cells and cross-presenting antigens, and they determine the type of immune response elicited against an antigen. TAT peptide (TATp), is an amphipathic, arginine-rich, cationic peptide that promotes penetration and translocation of various molecules and nanoparticles into cells. TATp-liposomes (TATp-L) used for DC transfection were prepared using TATp derivatized with a lipid-terminated polymer capable of anchoring in the liposomal membrane. Here, we show that the addition of TATp to DNA-loaded liposomes increased the uptake of DNA in DC. DNA-loaded TATp-L increased the in vitro transfection efficiency in DC cultures as evidenced by a higher expression of the enhanced green fluorescent protein and bovine herpes virus type 1 glycoprotein D (gD). The de novo synthesized gD protein was immunologically stimulating when transfections were performed with TATp-L, as indicated by the secretion of interleukin 6.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fragmentos de Peptídeos
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Células Dendríticas
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Transfecção
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Produtos do Gene tat do Vírus da Imunodeficiência Humana
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Lipossomos
Limite:
Animals
Idioma:
En
Revista:
Int J Nanomedicine
Ano de publicação:
2014
Tipo de documento:
Article