Expression, purification and crystallization of a dye-decolourizing peroxidase from Dictyostelium discoideum.
Acta Crystallogr F Struct Biol Commun
; 70(Pt 2): 252-5, 2014 Feb.
Article
em En
| MEDLINE
| ID: mdl-24637768
ABSTRACT
Dye-decolourizing peroxidases are haem-containing peroxidases with broad substrate specificity. Using H2O2 as an electron acceptor, they efficiently decolourize various dyes that are of industrial and environmental relevance, such as anthraquninone- and azo-based dyes. In this study, the dye-decolourizing peroxidase DdDyP from Dictyostelium discoideum was overexpressed in Escherichia coli strain Rosetta(DE3)pLysS, purified and crystallized using the vapour-diffusion method. A native crystal diffracted to 1.65 Å resolution and belonged to space group P4(1)2(1)2, with unit-cell parameters a = b = 141.03, c = 95.56 Å, α = ß = γ = 90°. The asymmetric unit contains two molecules.
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1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Peroxidases
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Cor
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Cristalografia por Raios X
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Dictyostelium
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Corantes
Idioma:
En
Revista:
Acta Crystallogr F Struct Biol Commun
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
Alemanha