Crystal structures of Pseudomonas putida esterase reveal the functional role of residues 187 and 287 in substrate binding and chiral recognition.

Dou, Shuai; Kong, Xu-Dong; Ma, Bao-Di; Chen, Qi; Zhang, Jie; Zhou, Jiahai; Xu, Jian-He

Dou, Shuai; Kong, Xu-Dong; Ma, Bao-Di; Chen, Qi; Zhang, Jie; Zhou, Jiahai; Xu, Jian-He.

Afiliação

Dou S; State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China; Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032, China.
Kong XD; State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China; Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032, China.
Ma BD; State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China.
Chen Q; State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China.
Zhang J; State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China.
Zhou J; Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032, China; Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Wuhan University, Wuhan 430071, China. Electronic address: jiahai@sioc.ac.cn.
Xu JH; State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China. Electronic address: jianhexu@ecust.edu.cn.

Biochem Biophys Res Commun ; 446(4): 1145-50, 2014 Apr 18.

Article em En | MEDLINE | ID: mdl-24680822

Assuntos

Carboxilesterase/química; Pseudomonas putida/enzimologia; Sítios de Ligação; Carboxilesterase/genética; Carboxilesterase/metabolismo; Domínio Catalítico; Cristalografia por Raios X; Modelos Moleculares; Mutação Puntual; Conformação Proteica; Pseudomonas putida/química; Pseudomonas putida/genética; Pseudomonas putida/metabolismo; Proteínas Recombinantes/química; Proteínas Recombinantes/genética; Proteínas Recombinantes/metabolismo; Especificidade por Substrato

Palavras-chave

Activity enhancement; Chiral recognition; Crystal structure; Enzyme-substrate complex; Protein engineering; Pseudomonas putida esterase

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pseudomonas putida / Carboxilesterase Idioma: En Revista: Biochem Biophys Res Commun Ano de publicação: 2014 Tipo de documento: Article País de afiliação: China País de publicação: Estados Unidos

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