Preliminary crystallographic analysis of the ankyrin-repeat domain of Arabidopsis thaliana AKT1: identification of the domain boundaries for protein crystallization.
Acta Crystallogr F Struct Biol Commun
; 70(Pt 4): 509-12, 2014 Apr.
Article
em En
| MEDLINE
| ID: mdl-24699751
ABSTRACT
The Arabidopsis thaliana K(+) transporter 1 (AKT1) participates in the maintenance of an adequate cell potassium (K(+)) concentration. The CBL-interacting protein kinase 23 (CIPK23) activates AKT1 for K(+) uptake under low-K(+) conditions. This process is mediated by the interaction between the cytosolic ankyrin-repeat (AR) domain of AKT1 and the kinase domain of CIPK23. However, the precise boundaries of the AR domain and the residues responsible for the interaction are still unknown. Here, the optimization procedure to obtain an AR domain construct suitable for crystallization and the preliminary crystallographic analysis of the obtained crystals are reported. The crystals belonged to space group P21212, with unit-cell parameters a = 34.83, b = 65.89, c = 85.44â
Å, and diffracted to 1.98â
Å resolution.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Canais de Potássio
/
Arabidopsis
/
Repetição de Anquirina
/
Cristalografia por Raios X
/
Proteínas de Arabidopsis
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Acta Crystallogr F Struct Biol Commun
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
Espanha