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Mucilaginibacter pineti sp. nov., isolated from Pinus pinaster wood from a mixed grove of pines trees.
Paiva, Gabriel; Abreu, Pedro; Proença, Diogo Neves; Santos, Susana; Nobre, Maria Fernanda; Morais, Paula V.
Afiliação
  • Paiva G; IMAR-CMA, University of Coimbra, 3004-517 Coimbra, Portugal.
  • Abreu P; IMAR-CMA, University of Coimbra, 3004-517 Coimbra, Portugal.
  • Proença DN; IMAR-CMA, University of Coimbra, 3004-517 Coimbra, Portugal.
  • Santos S; IMAR-CMA, University of Coimbra, 3004-517 Coimbra, Portugal.
  • Nobre MF; CNC-Center for Neuroscience and Cell Biology, University of Coimbra, 3004-517 Coimbra, Portugal.
  • Morais PV; Department of Life Sciences, FCTUC, University of Coimbra, 3004-517 Coimbra, Portugal.
Int J Syst Evol Microbiol ; 64(Pt 7): 2223-2228, 2014 Jul.
Article em En | MEDLINE | ID: mdl-24711588
Bacterial strain M47C3B(T) was isolated from the endophytic microbial community of a Pinus pinaster tree branch from a mixed grove of pines. Phylogenetic analysis of 16S rRNA gene sequences showed that this organism represented one distinct branch within the family Sphingobacteriaceae, most closely related to the genus Mucilaginibacter. Strain M47C3B(T) formed a distinct lineage, closely related to Mucilaginibacter dorajii KACC 14556(T), with which it shared 97.2% 16S rRNA gene sequence similarity. The other members of the genus Mucilaginibacter included in the same clade were Mucilaginibacter lappiensis ATCC BAA-1855(T) sharing 97.0% similarity and Mucilaginibacter composti TR6-03(T) that had a lower similarity (95.7%). The novel strain was Gram-staining-negative, formed rod-shaped cells, grew optimally at 26 °C and at pH 7, and was able to grow with up to 0.3% (w/v) NaCl. The respiratory quinone was menaquinone 7 (MK-7) and the major fatty acids of the strain were summed feature 3 (C16 : 1ω7c/iso-C15 : 0 2-OH), iso-C15 : 0 and iso-C17 : 0 3-OH, representing 73.5% of the total fatty acids. The major components of the polar lipid profile of strain M47C3B(T) consisted of phosphatidylethanolamine, three unidentified aminophospholipids, one unidentified aminolipid and three unidentified polar lipids. The G+C content of the DNA was 40.6 mol%. On the basis of the phylogenetic analysis and physiological and biochemical characteristics we propose the name Mucilaginibacter pineti sp. nov. for the novel species represented by strain M47C3B(T) ( = CIP 110632(T) = LMG 28160(T)).
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Filogenia / Madeira / Pinus / Bacteroidetes País/Região como assunto: Europa Idioma: En Revista: Int J Syst Evol Microbiol Assunto da revista: MICROBIOLOGIA Ano de publicação: 2014 Tipo de documento: Article País de afiliação: Portugal País de publicação: Reino Unido

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Filogenia / Madeira / Pinus / Bacteroidetes País/Região como assunto: Europa Idioma: En Revista: Int J Syst Evol Microbiol Assunto da revista: MICROBIOLOGIA Ano de publicação: 2014 Tipo de documento: Article País de afiliação: Portugal País de publicação: Reino Unido