[Effects of docosahexaenoic acid on hypoxia-induced pulmonary arterial hypertension].
Zhonghua Jie He He Hu Xi Za Zhi
; 37(2): 109-12, 2014 Feb.
Article
em Zh
| MEDLINE
| ID: mdl-24796591
ABSTRACT
OBJECTIVE:
To investigate the effects of docosahexaenoic acid (DHA) on hypoxia-induced pulmonary arterial hypertension(PAH) and the mechanism.METHODS:
PAH was induced by chronic intermittent hypoxia for 21 days in vivo. Forty male Sprague-Dawley rats were randomly divided into 4 groups (n = 10, each)a normal control group, DHA-treated groups in normoxia and hypoxia, and a PAH group. At the end of study, mean pulmonary arterial pressure (mPAP), right ventricular hypertrophy and the index of wall thickness of small pulmonary artery (WT% and WA%) among groups were compared. The changes of pulmonary arterial smooth muscle cell (PASMC) proliferation were determined by MTT in vitro. Migration assay was performed using the Boyden chamber. Real-time quantitative PCR was performed to quantify mRNA levels of the smooth muscle cell phenotype markers SM-α-actin, calponin and SM 22α under normoxic or hypoxic conditions, in the absence or presence of DHA.RESULTS:
DHA treatment significantly lowered mPAP [(22.7 ± 1.8) mmHg (1 mmHg = 0.133 kPa)], reduced thickening of small pulmonary artery wall [WT%(21.6 ± 4.1)%, WA% (52.0 ± 2.9)% ] and alleviated ventricular hypertrophy (34.2 ± 2.2) % compared to those of the hypoxic group (P < 0.05). DHA inhibited the proliferation, migration and phenotype switching of PASMCs induced by hypoxia in vitro.CONCLUSION:
DHA therapy reduced mPAP in a rat model of hypoxia-induced PAH and this effect was linked with inhibition of pulmonary vascular remodelling.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Artéria Pulmonar
/
Ácidos Docosa-Hexaenoicos
/
Miócitos de Músculo Liso
/
Hipertensão Pulmonar
/
Hipóxia
Tipo de estudo:
Etiology_studies
Limite:
Animals
Idioma:
Zh
Revista:
Zhonghua Jie He He Hu Xi Za Zhi
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
China