Your browser doesn't support javascript.
loading
Investigation of IGF2, Hedgehog and fusion gene expression profiles in pediatric sarcomas.
de Souza, Robson Ramos; Oliveira, Indhira Dias; del Giúdice Paniago, Mario; Yaoita, Fernando Hideki Kato; Caran, Eliana Maria Monteiro; Macedo, Carla Renata Pacheco Donato; Petrilli, Antonio Sergio; Abib, Simone de Campos Vieira; de Seixas Alves, Maria Teresa; de Toledo, Silvia Regina Caminada.
Afiliação
  • de Souza RR; Pediatric Oncology Institute (GRAACC), Department of Pediatrics, Federal University of São Paulo, São Paulo, SP, Brazil; Department of Structural and Functional Biology, Federal University of São Paulo, São Paulo, SP, Brazil. Electronic address: rramos.souza@hotmail.com.
  • Oliveira ID; Pediatric Oncology Institute (GRAACC), Department of Pediatrics, Federal University of São Paulo, São Paulo, SP, Brazil; Department of Structural and Functional Biology, Federal University of São Paulo, São Paulo, SP, Brazil. Electronic address: indhiradias@graacc.org.br.
  • del Giúdice Paniago M; Pediatric Oncology Institute (GRAACC), Department of Pediatrics, Federal University of São Paulo, São Paulo, SP, Brazil. Electronic address: mariopaniago@graacc.org.br.
  • Yaoita FH; Pediatric Oncology Institute (GRAACC), Department of Pediatrics, Federal University of São Paulo, São Paulo, SP, Brazil; Department of Structural and Functional Biology, Federal University of São Paulo, São Paulo, SP, Brazil. Electronic address: fernando.yaoita@gmail.com.
  • Caran EM; Pediatric Oncology Institute (GRAACC), Department of Pediatrics, Federal University of São Paulo, São Paulo, SP, Brazil. Electronic address: elianacaran@graacc.org.br.
  • Macedo CR; Pediatric Oncology Institute (GRAACC), Department of Pediatrics, Federal University of São Paulo, São Paulo, SP, Brazil. Electronic address: carladonatomacedo@uol.com.br.
  • Petrilli AS; Pediatric Oncology Institute (GRAACC), Department of Pediatrics, Federal University of São Paulo, São Paulo, SP, Brazil. Electronic address: sergiopetrilli@graacc.org.br.
  • Abib Sde C; Pediatric Oncology Institute (GRAACC), Department of Pediatrics, Federal University of São Paulo, São Paulo, SP, Brazil; Division of Pediatric Surgery, Federal University of São Paulo, São Paulo, SP, Brazil. Electronic address: simoneabib@uol.com.br.
  • de Seixas Alves MT; Pediatric Oncology Institute (GRAACC), Department of Pediatrics, Federal University of São Paulo, São Paulo, SP, Brazil; Department of Pathology, Federal University of São Paulo, São Paulo, SP, Brazil. Electronic address: mtseixas@patologia.epm.br.
  • de Toledo SR; Pediatric Oncology Institute (GRAACC), Department of Pediatrics, Federal University of São Paulo, São Paulo, SP, Brazil; Department of Structural and Functional Biology, Federal University of São Paulo, São Paulo, SP, Brazil. Electronic address: silviatoledo@graacc.org.br.
Growth Horm IGF Res ; 24(4): 130-6, 2014 Aug.
Article em En | MEDLINE | ID: mdl-24846856
ABSTRACT
UNLABELLED The childhood sarcomas are malignant tumors with high mortality rates. They are divided into two genetic categories a category without distinct pattern karyotypic changes and the other category showing unique translocations that originate gene rearrangements. This category includes rhabdomyosarcoma (RMS), Ewing's sarcoma (ES) and synovial sarcoma (SS). Diverse studies have related development genes, such as; IGF2, IHH, PTCH1 and GLI1 and sarcomatogenesis.

OBJECTIVE:

To characterize the RMS, ES and SS rearrangements, we quantify the expression of IGF2 IHH, PTCH1 and GLI1 genes and correlate molecular data with clinical parameters of patients.

DESIGN:

We analyzed 29 RMS, 10 SS and 60 ES tumor samples by RT-PCR (polymerase chain reaction-reverse transcription) and qPCR (quantitative PCR).

RESULTS:

Among the samples of ARMS, 50% had rearrangements of PAX3/7-FOXO1, 60% of ES samples were EWS-FLI1 positive and 90% of SS samples were positive for SS18-SSX1/2. In relation to the control reference samples (QPCR Human Reference Total RNA-Stratagene, Human Skeletal Muscle Total RNA-Ambion, Universal RNA Human Normal Tissues-Ambion), RMS samples showed a high IGF2 gene expression (p<0.0001). Moreover, ES samples showed a low IGF2 gene expression (p<0.0001) and high IHH (p<0.0001), PTCH1 (p=0.0173) and GLI1 (p=0.0113) gene expressions.

CONCLUSIONS:

The molecular characterization of IGF and Hedgehog pathway in these pediatric sarcomas may collaborate to enable a better understanding of the biological behavior of these neoplasms.
Assuntos
Palavras-chave

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Sarcoma / Fator de Crescimento Insulin-Like II / Regulação Neoplásica da Expressão Gênica / Proteínas de Fusão Oncogênica / Proteínas Hedgehog Limite: Adolescent / Adult / Child / Child, preschool / Female / Humans / Infant / Male Idioma: En Revista: Growth Horm IGF Res Assunto da revista: ENDOCRINOLOGIA Ano de publicação: 2014 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Sarcoma / Fator de Crescimento Insulin-Like II / Regulação Neoplásica da Expressão Gênica / Proteínas de Fusão Oncogênica / Proteínas Hedgehog Limite: Adolescent / Adult / Child / Child, preschool / Female / Humans / Infant / Male Idioma: En Revista: Growth Horm IGF Res Assunto da revista: ENDOCRINOLOGIA Ano de publicação: 2014 Tipo de documento: Article
...