Kinetics of oxygen uptake by cells potentially used in a tissue engineered trachea.
Biomaterials
; 35(25): 6829-37, 2014 Aug.
Article
em En
| MEDLINE
| ID: mdl-24875760
ABSTRACT
Synthetic polymer scaffold seeded with autologous cells have a clinical translational potential. A rational design oriented to clinical applications must ensure an efficient mass transfer of nutrients as a function of specific metabolic rates, especially for precariously vascularized tissues grown in vitro or integrated in vivo. In this work, luminescence lifetime-based sensors were used to provide accurate, extensive and non-invasive measurements of the oxygen uptake rate for human mesenchymal stem cells (hMSCs), tracheal epithelial cells (hTEpiCs) and human chondrocytes (hCCs) within a range of 2-40% O2 partial pressure. Estimated Michaelis-Menten parameters were V(max) = 0.099 pmol/cellâ
h and K(M) = 2.12 × 10(-7) mol/cm(3) for hMSCs, V(max) = 1.23 pmol/cellâ
h and K(M) = 2.14 × 10(-7) mol/cm(3) for hTEpiCs, V(max) = 0.515 pmol/cellâ
h and K(M) = 1.65 × 10(-7) mol/cm(3) for hCCs. Kinetics data served as an input to a preliminary computational simulation of cell culture on a poly-ethylene terephthalate (PET) tracheal scaffold obtaining an efficient mass transfer at cell density of 10(6) cell/cm(3). Oxygen concentration affected the glucose uptake and lactate production rates of cells that adapted their metabolism according to energy demand in hypoxic and normoxic conditions.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oxigênio
/
Polímeros
/
Traqueia
/
Engenharia Tecidual
Limite:
Humans
Idioma:
En
Revista:
Biomaterials
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
Itália