Immunisation with bacterial expressed VP2 and VP5 of bluetongue virus (BTV) protect α/ß interferon-receptor knock-out (IFNAR(-/-)) mice from homologous lethal challenge.
Vaccine
; 32(32): 4059-67, 2014 Jul 07.
Article
em En
| MEDLINE
| ID: mdl-24886956
ABSTRACT
BTV-4 structural proteins VP2 (as two domains VP2D1 and VP2D2), VP5 (lacking the first 100 amino acids VP5Δ1-100) and full-length VP7, expressed in bacteria as soluble glutathione S-transferase (GST) fusion-proteins, were used to immunise Balb/c and α/ß interferon receptor knock-out (IFNAR(-/-)) mice. Neutralising antibody (NAbs) titres (expressed as log10 of the reciprocal of the last dilution of mouse serum which reduced plaque number by ≥50%) induced by the VP2 domains ranged from 1.806 to 2.408 in Balb/c and IFNAR(-/-) mice. The immunised IFNAR(-/-) mice challenged with a homologous live BTV-4 survived and failed to develop signs of infection (ocular discharge and apathy). Although subsequent attempts to isolate virus were unsuccessful (possibly reflecting presence of neutralising antibodies), a transient/low level viraemia was detected by real time RT-PCR. In contrast, mice immunised with the two VP2 domains with or without VP5Δ1-100 and VP7, then challenged with the heterologous serotype, BTV-8, all died by day 7 post-infection. We conclude that immunisation with bacterially-expressed VP2 domains can induce strong serotype-specific NAb responses. Bacterial expression could represent a cost effective and risk-free alternative to the use of live or inactivated vaccines, particularly if viruses prove to be difficult to propagate in cell culture (like BTV-25). A vaccine based on bacterially expressed VP2 and VP5 of BTV is also DIVA-compatible.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vacinas Virais
/
Bluetongue
/
Proteínas do Capsídeo
/
Receptor de Interferon alfa e beta
Limite:
Animals
Idioma:
En
Revista:
Vaccine
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
Reino Unido