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Development and evaluation of the rVP-ELISA for detection of antibodies against porcine parvovirus.
Kong, Miaomiao; Peng, Yonggang; Cui, Yuchao; Chang, Tiecheng; Wang, Xiaoling; Liu, Zhaoxia; Liu, Yonggang; Zhu, Yu; Luo, Yakun; Tang, Qinghai; Feng, Li; Cui, Shangjin.
Afiliação
  • Kong M; State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, 427 Maduan Street, Nangang District, Harbin 150001, Heilongjiang, China; Institute of Animal Sciences (IAS), Chinese Academy of Agricultural Sciences, Beijing 100094, C
  • Peng Y; State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, 427 Maduan Street, Nangang District, Harbin 150001, Heilongjiang, China.
  • Cui Y; State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, 427 Maduan Street, Nangang District, Harbin 150001, Heilongjiang, China; College of Animal Science and Technology, HLJ August First Land Reclamation University, Daqing
  • Chang T; State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, 427 Maduan Street, Nangang District, Harbin 150001, Heilongjiang, China; College of Animal Science and Technology, HLJ August First Land Reclamation University, Daqing
  • Wang X; State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, 427 Maduan Street, Nangang District, Harbin 150001, Heilongjiang, China.
  • Liu Z; State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, 427 Maduan Street, Nangang District, Harbin 150001, Heilongjiang, China.
  • Liu Y; State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, 427 Maduan Street, Nangang District, Harbin 150001, Heilongjiang, China.
  • Zhu Y; State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, 427 Maduan Street, Nangang District, Harbin 150001, Heilongjiang, China; Institute of Animal Sciences (IAS), Chinese Academy of Agricultural Sciences, Beijing 100094, C
  • Luo Y; State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, 427 Maduan Street, Nangang District, Harbin 150001, Heilongjiang, China; Institute of Animal Sciences (IAS), Chinese Academy of Agricultural Sciences, Beijing 100094, C
  • Tang Q; Nanyang Normal University, 1638 Wolong Road, Nanyang 473061, China.
  • Feng L; State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, 427 Maduan Street, Nangang District, Harbin 150001, Heilongjiang, China.
  • Cui S; State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, 427 Maduan Street, Nangang District, Harbin 150001, Heilongjiang, China; Institute of Animal Sciences (IAS), Chinese Academy of Agricultural Sciences, Beijing 100094, C
J Virol Methods ; 206: 115-8, 2014 Sep.
Article em En | MEDLINE | ID: mdl-24945904
ABSTRACT
The gene encoding the VP2 protein of porcine parvovirus (PPV) was expressed in an insect-baculovirus system. The recombinant (r) VP2 was similar antigenically/functionally to the native capsid protein as demonstrated by hemagglutination (HA), Western blotting using PPV positive sera. The purified rVP2 proteins were used as coating antigen to establish a rVP-ELISA method for detection of PPV positive and negative sera from pigs. The optimal operating conditions of the rVP-ELISA were the concentration of rVP2 proteins coated on the wells was 2 µg/mL; the diluted concentration of serum was 1 150 and that of the enzyme-labeled antibody was 1 6000. A total of 596 sera were detected by this assay, and the average positive rate was 87%. Compared with France LSI kit, the result showed that the coincidence rate was 96.7%. In conclusion, the rVP2-ELISA is a sensitive and specific method for detecting antibodies against PPV.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Doenças dos Suínos / Medicina Veterinária / Infecções por Parvoviridae / Parvovirus Suíno / Proteínas do Capsídeo / Anticorpos Antivirais / Antígenos Virais Tipo de estudo: Diagnostic_studies / Evaluation_studies Limite: Animals Idioma: En Revista: J Virol Methods Ano de publicação: 2014 Tipo de documento: Article
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Doenças dos Suínos / Medicina Veterinária / Infecções por Parvoviridae / Parvovirus Suíno / Proteínas do Capsídeo / Anticorpos Antivirais / Antígenos Virais Tipo de estudo: Diagnostic_studies / Evaluation_studies Limite: Animals Idioma: En Revista: J Virol Methods Ano de publicação: 2014 Tipo de documento: Article