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Genetic and biological characterization of selected Changuinola viruses (Reoviridae, Orbivirus) from Brazil.
Silva, Sandro P; Dilcher, Meik; Weber, Franziska; Hufert, Frank T; Weidmann, Manfred; Cardoso, Jedson F; Carvalho, Valéria L; Chiang, Jannifer O; Martins, Lívia C; Lima, Clayton P S; Da Silva, Daisy E A; Vianez-Júnior, João L S G; Popov, Vsevolod L; Travassos da Rosa, Amélia P A; Tesh, Robert B; Vasconcelos, Pedro F C; Nunes, Márcio R T.
Afiliação
  • Silva SP; Center for Technological Innovation of Evandro Chagas Institute, Ministry of Health, BR 316, Km 07, s/n CEP 67.030-000, Ananindeua, Brazil.
  • Dilcher M; Department of Virology, University Medical Center Göttingen, Kreuzbergring 57, D-37075 Göttingen, Germany.
  • Weber F; Department of Virology, University Medical Center Göttingen, Kreuzbergring 57, D-37075 Göttingen, Germany.
  • Hufert FT; Department of Virology, University Medical Center Göttingen, Kreuzbergring 57, D-37075 Göttingen, Germany.
  • Weidmann M; Institute of Aquaculture, University of Stirling, Stirling FK9 4LA, UK.
  • Cardoso JF; Center for Technological Innovation of Evandro Chagas Institute, Ministry of Health, BR 316, Km 07, s/n CEP 67.030-000, Ananindeua, Brazil.
  • Carvalho VL; Departamento de Arbovirologia e Febres Hemorrágicas, Instituto Evandro Chagas, Ministério da Saúde, BR 316, Km 07, s/n CEP 67.030-000, Ananindeua, Brazil.
  • Chiang JO; Departamento de Arbovirologia e Febres Hemorrágicas, Instituto Evandro Chagas, Ministério da Saúde, BR 316, Km 07, s/n CEP 67.030-000, Ananindeua, Brazil.
  • Martins LC; Departamento de Arbovirologia e Febres Hemorrágicas, Instituto Evandro Chagas, Ministério da Saúde, BR 316, Km 07, s/n CEP 67.030-000, Ananindeua, Brazil.
  • Lima CPS; Center for Technological Innovation of Evandro Chagas Institute, Ministry of Health, BR 316, Km 07, s/n CEP 67.030-000, Ananindeua, Brazil.
  • Da Silva DEA; Center for Technological Innovation of Evandro Chagas Institute, Ministry of Health, BR 316, Km 07, s/n CEP 67.030-000, Ananindeua, Brazil.
  • Vianez-Júnior JLSG; Center for Technological Innovation of Evandro Chagas Institute, Ministry of Health, BR 316, Km 07, s/n CEP 67.030-000, Ananindeua, Brazil.
  • Popov VL; Department of Pathology, Center for Biodefense and Emerging Infectious Diseases, University of Texas Medical Branch, Galveston, TX 77555, USA.
  • Travassos da Rosa APA; Department of Pathology, Center for Biodefense and Emerging Infectious Diseases, University of Texas Medical Branch, Galveston, TX 77555, USA.
  • Tesh RB; Department of Pathology, Center for Biodefense and Emerging Infectious Diseases, University of Texas Medical Branch, Galveston, TX 77555, USA.
  • Vasconcelos PFC; Departamento de Patologia, Universidade do Estado do Pará, Belém, Brazil.
  • Nunes MRT; Departamento de Arbovirologia e Febres Hemorrágicas, Instituto Evandro Chagas, Ministério da Saúde, BR 316, Km 07, s/n CEP 67.030-000, Ananindeua, Brazil.
J Gen Virol ; 95(Pt 10): 2251-2259, 2014 Oct.
Article em En | MEDLINE | ID: mdl-24986085
ABSTRACT
The genus Orbivirus of the family Reoviridae comprises 22 virus species including the Changuinola virus (CGLV) serogroup. The complete genome sequences of 13 CGLV serotypes isolated between 1961 and 1988 from distinct geographical areas of the Brazilian Amazon region were obtained. All viral sequences were obtained from single-passaged CGLV strains grown in Vero cells. CGLVs are the only orbiviruses known to be transmitted by phlebotomine sandflies. Ultrastructure and molecular analysis by electron microscopy and gel electrophoresis, respectively, revealed viral particles with typical orbivirus size and morphology, as well as the presence of a segmented genome with 10 segments. Full-length nucleotide sequencing of each of the ten RNA segments of the 13 CGLV serotypes provided basic information regarding the genome organization, encoded proteins and genetic traits. Segment 2 (encoding VP2) of the CGLV is uncommonly larger in comparison to those found in other orbiviruses and shows varying sizes even among different CGLV serotypes. Phylogenetic analysis support previous serological findings, which indicate that CGLV constitutes a separate serogroup within the genus Orbivirus. In addition, six out of 13 analysed CGLV serotypes showed reassortment of their genome segments.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: RNA Viral / Genoma Viral / Análise de Sequência de DNA / Orbivirus Limite: Animals / Humans País/Região como assunto: America do sul / Brasil Idioma: En Revista: J Gen Virol Ano de publicação: 2014 Tipo de documento: Article País de afiliação: Brasil
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: RNA Viral / Genoma Viral / Análise de Sequência de DNA / Orbivirus Limite: Animals / Humans País/Região como assunto: America do sul / Brasil Idioma: En Revista: J Gen Virol Ano de publicação: 2014 Tipo de documento: Article País de afiliação: Brasil