Enamel matrix derivative protein enhances production of matrixmetalloproteinase-2 by osteoblasts.
BMC Oral Health
; 14: 85, 2014 Jul 10.
Article
em En
| MEDLINE
| ID: mdl-25011999
ABSTRACT
BACKGROUND:
Matrix metalloproteinases (MMPs) degrade the extracellular matrix (ECM) and regulate remodeling and regeneration of bone. Enamel matrix derivative (EMD) protein has been used clinically for periodontal regeneration, although its molecular mechanisms are not clear. We evaluated the role of matrix metalloproteinases (MMPs) in regulating EMD-dependent degradation of gelatin on oeoblast-like cell line MG63.METHODS:
MG-63 cells (osteoblast cell line) were incubated with 100 µg/ml EMD protein in the presence or absence of MMP-2 tissue inhibitor for 20 h followed by incubation on DQ-gelatin-coated plates for 4 h. MG-63 cells (1 × 10(6)) were preincubated with SB203580 for 30 min at 37°C and were then placed in 100 µg/ml EMD protein for 24 h. Conditioned media were collected and detected by Western blot analysis.RESULTS:
EMD protein enhanced cell-mediated degradation of gelatin, which was inhibited by the MMP inhibitor TIMP-2. Furthermore, MMP-2 was produced by MG63 cells in response to EMD protein in a P38 MAPK-dependent manner. In addition, blocking of p38 MAPK activation by SB203580 significantly inhibited generation of the active form of MMP-2.CONCLUSION:
P38 MAPK pathway promotes expression MMP-2 in EMD activated osteoblasts, which in turn stimulates periodontal regeneration by degrading matrix proteins in periodontal connective tissue.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Osteoblastos
/
Metaloproteinase 2 da Matriz
/
Proteínas do Esmalte Dentário
Limite:
Humans
Idioma:
En
Revista:
BMC Oral Health
Assunto da revista:
ODONTOLOGIA
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
Japão