Extraction and purification of C-phycocyanin from Spirulina platensis (CCC540).
Indian J Plant Physiol
; 19: 184-188, 2014.
Article
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| ID: mdl-25089058
ABSTRACT
In this study a simple protocol was developed for purifying phycocyanin (PC) from Spirulina platensis (CCC540) by using ammonium sulphate precipitation, followed by a single step chromatography by using DEAE-Cellulose-11 and acetate buffer. Precipitation with 65 % ammonium sulphate resulted in 80 % recovery of phycocyanin with purity of 1.5 (A620/A280). Thro1ugh chromatography an 80 % recovery of phycocyanin with a purity of 4.5 (A620/A280) was achieved. In SDS_PAGE analysis, the purified PC showed the presence of two subunit α (16 kD) and ß (17 kD).
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01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
Indian J Plant Physiol
Ano de publicação:
2014
Tipo de documento:
Article