Extraction and purification of C-phycocyanin from <i>Spirulina platensis</i> (CCC540).

Kumar, Devendra; Dhar, Dolly Wattal; Pabbi, Sunil; Kumar, Neeraj; Walia, Suresh

Extraction and purification of C-phycocyanin from Spirulina platensis (CCC540).

Kumar, Devendra; Dhar, Dolly Wattal; Pabbi, Sunil; Kumar, Neeraj; Walia, Suresh.

Afiliação

Kumar D; Centre for Conservation and Utilisation of Blue Green Algae, Indian Agricultural Research Institute, New Delhi, 110012 India.
Dhar DW; Centre for Conservation and Utilisation of Blue Green Algae, Indian Agricultural Research Institute, New Delhi, 110012 India.
Pabbi S; Centre for Conservation and Utilisation of Blue Green Algae, Indian Agricultural Research Institute, New Delhi, 110012 India.
Kumar N; Microbiology Department, Kurukshetra University, Kurukshetra, India.
Walia S; Division of Agricultural Chemical, Indian Agricultural Research Institute, New Delhi, India.

Indian J Plant Physiol ; 19: 184-188, 2014.

Article em En | MEDLINE | ID: mdl-25089058

ABSTRACT

ABSTRACT

In this study a simple protocol was developed for purifying phycocyanin (PC) from Spirulina platensis (CCC540) by using ammonium sulphate precipitation, followed by a single step chromatography by using DEAE-Cellulose-11 and acetate buffer. Precipitation with 65 % ammonium sulphate resulted in 80 % recovery of phycocyanin with purity of 1.5 (A620/A280). Thro1ugh chromatography an 80 % recovery of phycocyanin with a purity of 4.5 (A620/A280) was achieved. In SDS_PAGE analysis, the purified PC showed the presence of two subunit α (16 kD) and ß (17 kD).

Palavras-chave

DEAE-Cellulose-11; Phycocyanin; Spirulina

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Indian J Plant Physiol Ano de publicação: 2014 Tipo de documento: Article