Reduction of nitromin to nitrogen mustard: unscheduled DNA synthesis in aerobic or anaerobic rat hepatocytes, JB1, BL8 and Walker carcinoma cell lines.
Carcinogenesis
; 10(11): 2113-8, 1989 Nov.
Article
em En
| MEDLINE
| ID: mdl-2509092
ABSTRACT
A novel route for the microsomal generation of nitrogen mustard from its N-oxide nitromin is demonstrated. The mustard was trapped as an adduct with diethyldithiocarbamate and estimated by capillary GLC. The enzyme responsible for this reduction could utilize either NADPH or NADH. Reduction occurred preferentially under anaerobic conditions. Purified cytochrome P450 reductase could carry out this reaction. Similar activities were seen using microsomal fractions from rat liver or liver derived BL8, JB1 or Walker 256 carcinoma cells, when these were expressed on a per mg of protein basis. Unscheduled DNA synthesis (UDS) was used as an index of activation of nitromin in these cell systems. In all instances, greater induction of UDS occurred in cells incubated with nitromin under anaerobic conditions.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Reparo do DNA
/
Mecloretamina
/
Antineoplásicos
/
Compostos de Mostarda Nitrogenada
Limite:
Animals
Idioma:
En
Revista:
Carcinogenesis
Ano de publicação:
1989
Tipo de documento:
Article
País de afiliação:
Reino Unido