Your browser doesn't support javascript.
loading
A radioisotope-nondependent high-sensitivity method for measuring the activity of glioblastoma-related O(6)-methylguanine DNA methyltransferase.
Hongo, Aya; Gu, Ran; Suzuki, Miho; Nemoto, Naoto; Nishigaki, Koichi.
Afiliação
  • Hongo A; Department of Functional Materials Science, Graduate School of Science and Engineering, Saitama University, Sakura-ku, Saitama 338-8570, Japan.
  • Gu R; Department of Functional Materials Science, Graduate School of Science and Engineering, Saitama University, Sakura-ku, Saitama 338-8570, Japan.
  • Suzuki M; Department of Functional Materials Science, Graduate School of Science and Engineering, Saitama University, Sakura-ku, Saitama 338-8570, Japan; Rational Evolutionary Design of Advanced Biomolecules, Saitama (REDS), Saitama Small Enterprise Promotion Corporation, No. 552, Kawaguchi, Saitama 333-0844,
  • Nemoto N; Department of Functional Materials Science, Graduate School of Science and Engineering, Saitama University, Sakura-ku, Saitama 338-8570, Japan; Rational Evolutionary Design of Advanced Biomolecules, Saitama (REDS), Saitama Small Enterprise Promotion Corporation, No. 552, Kawaguchi, Saitama 333-0844,
  • Nishigaki K; Department of Functional Materials Science, Graduate School of Science and Engineering, Saitama University, Sakura-ku, Saitama 338-8570, Japan; Rational Evolutionary Design of Advanced Biomolecules, Saitama (REDS), Saitama Small Enterprise Promotion Corporation, No. 552, Kawaguchi, Saitama 333-0844,
Anal Biochem ; 480: 82-4, 2015 Jul 01.
Article em En | MEDLINE | ID: mdl-25173514
ABSTRACT
O(6)-Methylguanine DNA methyltransferase (MGMT) cancels the anticancer effect of temozolomide (drug for glioblastoma), which introduces methylation to DNA. Therefore, developing an MGMT inhibitor is a promising strategy for the treatment of this cancer. For this purpose, a sensitive detection method that does not depend on the conventional radioisotope (RI) method was developed. This was realized by a fluorescence-based method that measured the amount of cleavable restriction sites demethylated by the action of MGMT; this method was enhanced by introducing a polymerase chain reaction (PCR) amplification step. As an assay of enzyme activity, 20-fold higher sensitivity (subnanomolar) was attained compared with our and others' fluorescence-based approaches.
Assuntos
Palavras-chave
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Reação em Cadeia da Polimerase / Glioblastoma / O(6)-Metilguanina-DNA Metiltransferase / Fluorescência Tipo de estudo: Diagnostic_studies Limite: Humans Idioma: En Revista: Anal Biochem Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Japão
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Reação em Cadeia da Polimerase / Glioblastoma / O(6)-Metilguanina-DNA Metiltransferase / Fluorescência Tipo de estudo: Diagnostic_studies Limite: Humans Idioma: En Revista: Anal Biochem Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Japão