Purification and identification of ADP-ribosylated proteins from bull testis intact nuclei.
Biochem Int
; 19(6): 1265-75, 1989 Dec.
Article
em En
| MEDLINE
| ID: mdl-2517579
ABSTRACT
Isolated, intact bull testis nuclei were incubated with [14C] NAD. A large amount of radioactivity was associated to loosely bound chromosomal proteins extracted with 0.35M NaCl and fractionated with trichloroacetic acid. The labelled nuclear proteins included essentially a number of components belonging to the low mobility group. Mg2(+)-catalyzed alkali digestion of radioactive proteins and further analysis demonstrated that the final products were 5'-AMP and phospho-ribosyl-AMP, which arise from the hydrolysis of poly(ADP-ribose).
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Poli Adenosina Difosfato Ribose
/
Testículo
/
Proteínas Nucleares
/
Açúcares de Nucleosídeo Difosfato
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Revista:
Biochem Int
Ano de publicação:
1989
Tipo de documento:
Article
País de afiliação:
Itália