Protective effect of artichoke leaf extract against paracetamol-induced hepatotoxicity in rats.
Pharm Biol
; 53(2): 167-73, 2015 Feb.
Article
em En
| MEDLINE
| ID: mdl-25243875
CONTEXT: Paracetamol overdose is a predominant cause of hepatotoxicity in both humans and experimental animals. OBJECTIVE: In this study, we investigated the protective effect of aqueous artichoke leaf extract (ALE) against paracetamol-induced liver injury in rats using N-acetylcysteine (NAC) as a reference drug. MATERIALS AND METHODS: Rats were divided into five groups: negative control, paracetamol (2 g/kg, single oral dose), ALE (1.5 g/kg, orally for 14 d), ALE + paracetamol, and NAC (100 mg/kg) + paracetamol. Indices of liver damage (serum alanine aminotransferase and aspartate aminotransferase) were measured. Liver homogenates were analyzed for oxidative stress biomarkers (MDA, malondialdehyde; SOD activity, superoxide dismutase activity; NO, nitric oxide; GSH content, reduced glutathione), glutathione cycling (GR, glutathione reductase), and utilization (GST, glutathione-S-transferase). Apoptosis was assessed using the comet assay. RESULTS: Paracetamol caused marked liver damage as noted by significant increased activities of serum aminotransferases (p < 0.05) as well as a significant increase in hepatic MDA and NO levels (p < 0.001) compared with the negative control group. GSH content, GR, GST, and SOD activities were decreased significantly (p < 0.001). Comet assay parameters (tail length, percentage of tailed cells, percentage of migrated DNA, and tail moment) were increased (p < 0.05), indicating apoptosis. Histopathological examination showed necrotic areas. Pre-treatment with ALE replenished hepatic GSH, reversed oxidative stress parameters, DNA damage, and necrosis induced by paracetamol. DISCUSSION AND CONCLUSION: These results suggest that ALE may protect from paracetamol-induced liver toxicity via its antioxidant and anti-apoptotic properties.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Extratos Vegetais
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Cynara scolymus
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Doença Hepática Induzida por Substâncias e Drogas
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Acetaminofen
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Antioxidantes
Tipo de estudo:
Etiology_studies
Limite:
Animals
Idioma:
En
Revista:
Pharm Biol
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
Egito
País de publicação:
Reino Unido