Abi3bp regulates cardiac progenitor cell proliferation and differentiation.
Circ Res
; 115(12): 1007-16, 2014 Dec 05.
Article
em En
| MEDLINE
| ID: mdl-25296984
ABSTRACT
RATIONALE Cardiac progenitor cells (CPCs) are thought to differentiate into the major cell types of the heart cardiomyocytes, smooth muscle cells, and endothelial cells. We have recently identified ABI family, member 3 (NESH) binding protein (Abi3bp) as a protein important for mesenchymal stem cell biology. Because CPCs share several characteristics with mesenchymal stem cells, we hypothesized that Abi3bp would similarly affect CPC differentiation and proliferation. OBJECTIVE:
To determine whether Abi3bp regulates CPC proliferation and differentiation. METHODS ANDRESULTS:
In vivo, genetic ablation of the Abi3bp gene inhibited CPC differentiation, whereas CPC number and proliferative capacity were increased. This correlated with adverse recovery after myocardial infarction. In vitro, CPCs, either isolated from Abi3bp knockout mice or expressing an Abi3bp shRNA construct, displayed a higher proliferative capacity and, under differentiating conditions, reduced expression of both early and late cardiomyocyte markers. Abi3bp controlled CPC differentiation via integrin-ß1, protein kinase C-ζ, and v-akt murine thymoma viral oncogene homolog.CONCLUSIONS:
We have identified Abi3bp as a protein important for CPC differentiation and proliferation.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Transporte
/
Diferenciação Celular
/
Miócitos Cardíacos
/
Proliferação de Células
/
Células-Tronco Mesenquimais
/
Infarto do Miocárdio
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Circ Res
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
Nova Caledônia