Unfolded protein response prevents blastocyst formation during preimplantation embryo development in vitro.
Fertil Steril
; 102(6): 1777-84, 2014 Dec.
Article
em En
| MEDLINE
| ID: mdl-25305729
ABSTRACT
OBJECTIVE:
To study the effect of increased endoplasmic reticulum (ER) stress as a major nongenomic mechanism for arrested blastocyst development.DESIGN:
Cell and animal study.SETTING:
The Ohio State University and Yale University. ANIMAL(S) Mice. INTERVENTION(S) Pregnant mare serum gonadotropin and hCG were administered IP; two cell embryos were collected 48 hours after hCG administration. MAIN OUTCOME MEASURE(S) Blastocyst development rate. RESULT(S) No morphological difference was detected in control versus tunicamycin- (TM) treated embryos until the blastocyst stage. On day 4 of embryonic development, TM treatment reduced blastocyst formation from 79% to 4% and induced nuclear fragmentation. TM treatment caused 2-fold and 2.6-fold increase in binding immunoglobulin protein and spliced-X-box binding protein 1 mRNA expression, respectively. By comparison, the tauroursodeoxycholic acid + TM combination reversed the effect of TM alone on blastocyst formation to near control levels. CONCLUSION(S) These results indicate that increased ER stress during in vitro embryo development triggers an unfolded protein response (UPR) that negatively affects blastocyst formation and suggests that activation of UPR signaling may account for low rates of blastocyst development.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Blastocisto
/
Técnicas de Cultura Embrionária
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Desenvolvimento Embrionário
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Resposta a Proteínas não Dobradas
/
Estresse do Retículo Endoplasmático
Limite:
Animals
Idioma:
En
Revista:
Fertil Steril
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
Turquia