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[CD147 expression level and rs8259 T/A polymorphism of CD147 in patients with acute coronary syndrome].
Mao, Yu; Yan, Jinchuan; Wang, Cuiping; Wang, Zhongqun; Liu, Peijing; Yuan, Wei.
Afiliação
  • Mao Y; Department of Cardiology, Affiliated Hospital of Jiangsu University,Zhenjiang 212001, China.
  • Yan J; Department of Cardiology, Affiliated Hospital of Jiangsu University,Zhenjiang 212001, China. Email: yanjinchuan@hotmail.com.
  • Wang C; Department of Cardiology, Affiliated Hospital of Jiangsu University,Zhenjiang 212001, China.
  • Wang Z; Department of Cardiology, Affiliated Hospital of Jiangsu University,Zhenjiang 212001, China.
  • Liu P; Department of Cardiology, Affiliated Hospital of Jiangsu University,Zhenjiang 212001, China.
  • Yuan W; Department of Cardiology, Affiliated Hospital of Jiangsu University,Zhenjiang 212001, China.
Zhonghua Xin Xue Guan Bing Za Zhi ; 42(7): 566-70, 2014 Jul.
Article em Zh | MEDLINE | ID: mdl-25327598
OBJECTIVE: To investigate the association between CD147 expression and its untranslated regions 3'UTR rs8259 T/A polymorphism and acute coronary syndrome (ACS). METHODS: The genotypes of CD147 were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) methods in 182 ACS patients and 328 healthy controls. The plasma level of CD147 was determined by enzyme-linked immunosorbent assay (ELISA). CD147 mRNA and protein expression was detected by real-time fluorescent quantitative PCR (RT-qPCR) and Western blot. RESULTS: The plasma CD147 level obtained from radial artery in ACS patients ((3.63 ± 0.70) pg/L) was significantly higher than in control ((2.45 ± 0.27) pg/L, P < 0.05), and highest in plasma obtained from the coronary artery ((4.28 ± 1.03) pg/L, P < 0.05) in ACS patients. Furthermore, the plasma CD147 level was higher in the ACS patients with rs8259 AA genotype than in the ACS patients with rs8259 TT genotype ((4.08 ± 0.41) pg/L vs. (3.05 ± 0.79) pg/L in radial artery and (5.29 ± 0.62) pg/L vs. (3.13 ± 0.52) pg/L in coronary artery, both P < 0.05). There are an enhanced expression of CD147 mRNA (2.45 times higher than control) and protein (3.66 ± 1.56 vs. 1.81 ± 1.29) in PBMCs from ACS patients than that from controls (both P < 0.05). The PBMCs CD147 mRNA and protein expression level were significantly higher in ACS patients with rs8259 AA genotype (mRNA:2.45 ± 0.35, protein:1.63 ± 0.16) compared to ACS patients with rs8259 TT genotype (mRNA:1.69 ± 0.15, protein: 0.88 ± 0.16, both P < 0.05). Multiple logistic analysis showed that CD147 T allele (AT+TT) was a protective factor to ACS (OR = 0.667, 95% CI 0.507-0.879, P < 0.05). CONCLUSIONS: The over-expression of CD147 is involved in the pathogenesis of ACS. The CD147 3'UTR rs8259 T allele may be a protective factor for ACS, its polymorphism can affect the CD147 protein expression in ACS patients.
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Polimorfismo Genético / Basigina / Síndrome Coronariana Aguda Tipo de estudo: Observational_studies / Risk_factors_studies Limite: Humans Idioma: Zh Revista: Zhonghua Xin Xue Guan Bing Za Zhi Ano de publicação: 2014 Tipo de documento: Article País de afiliação: China País de publicação: China
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Polimorfismo Genético / Basigina / Síndrome Coronariana Aguda Tipo de estudo: Observational_studies / Risk_factors_studies Limite: Humans Idioma: Zh Revista: Zhonghua Xin Xue Guan Bing Za Zhi Ano de publicação: 2014 Tipo de documento: Article País de afiliação: China País de publicação: China