GABA(A) receptor overexpression in the lateral hypothalamic area attenuates gastric ischemiareperfusion injury in rats.
Mol Med Rep
; 11(2): 1057-62, 2015 Feb.
Article
em En
| MEDLINE
| ID: mdl-25354809
ABSTRACT
Excessive activation of the greater splanchnic nerve (GSN) has previously been determined to contribute to the progression of gastric ischemiareperfusion (GIR) injury. The present study was designed to estimate the protective effects of GABAA receptor (GABA(A)R) overexpression in the lateral hypothalamic area (LHA) against GIR injury. The GIR injury model was induced in rats by clamping the celiac artery for 30 min and then reperfusing for 1 h. Microinjection of recombinant adenoviral vectors overexpressing GABA(A)R (AdGABA(A)R) or control adenoviral vectors (AdCon) into the LHA was conducted in GIR and normal control rats. Significant protective effects were observed on day 2 after AdGABA(A)R treatment in the GIR injury rats. AdGABA(A)R treatment reduced plasma norepinephrine levels, plasma angiotensin II levels and peripheral GSN activity, but increased the gastric mucosal blood flow, as compared with AdCon treatment. These results indicate that adenoviral vectorinduced GABA(A)R overexpression in the LHA blunts GSN activity and subsequently alleviates the effects of gastric injury in GIR rats.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Traumatismo por Reperfusão
/
Receptores de GABA-A
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Região Hipotalâmica Lateral
Limite:
Animals
Idioma:
En
Revista:
Mol Med Rep
Ano de publicação:
2015
Tipo de documento:
Article