Inhibition of xanthine oxidase activity by gnaphalium affine extract.
Chin Med Sci J
; 29(4): 225-30, 2014 Dec.
Article
em En
| MEDLINE
| ID: mdl-25429747
ABSTRACT
OBJECTIVE:
To evaluate the inhibitory effect of Gnaphalium affine extracts on xanthine oxidase (XO) activity in vitro and to analyze the mechanism of this effect.METHODS:
In this in vitro study, Kinetic measurements were performed in 4 different inhibitor concentrations and 5 different xanthine concentrations (60, 100, 200, 300, 400 Μmol/L). Dixon and Lineweaver-Burk plot analysis were used to determine Ki values and the inhibition mode for the compounds isolated from Gnaphalium affine extract.RESULTS:
Four potent xanthine oxidase inhibitors were found in 95% ethanolic (v/v) Gnaphalium affine extract. Among them, the flavone Eupatilin exhibited the strongest inhibitory effect on XO with a inhibition constant (Ki) of 0.37 Μmol/L, lower than the Ki of allopurinol (4.56 mol/L), a known synthetic XO inhibitor. Apigenin (Ki of 0.56 Μmol/L, a proportion of 0.0053 in Gnaphalium affine), luteolin (Ki of 2.63 Μmol/L, 0.0032 in Gnaphalium affine) and 5-hydroxy-6,7,3',4'-tetramethoxyflavone (Ki of 3.15 Μmol/L, 0.0043 in Gnaphalium affine) also contributed to the inhibitory effect of Gnaphalium affine extract on XO activity.CONCLUSIONS:
These results suggest that the use of Gnaphalium affine in the treatment of gout could be attributed to its inhibitory effect on XO. This study provides a rational basis for the traditional use of Gnaphalium affine against gout.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Xantina Oxidase
/
Flavonoides
/
Gnaphalium
Idioma:
En
Revista:
Chin Med Sci J
Assunto da revista:
TERAPIAS COMPLEMENTARES
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
China