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Impedimetric method for measuring ultra-low E. coli concentrations in human urine.
Settu, Kalpana; Chen, Ching-Jung; Liu, Jen-Tsai; Chen, Chien-Lung; Tsai, Jang-Zern.
Afiliação
  • Settu K; Department of Electrical Engineering, National Central University, Jhongli, Taiwan.
  • Chen CJ; School of Electronic and Communication Engineering, University of Chinese Academy of Sciences, Beijing, China. Electronic address: me2452858@gmail.com.
  • Liu JT; College of Materials Sciences and Opto-Electronics, University of Chinese Academy of Sciences, Beijing, China. Electronic address: jentsai.liu@gmail.com.
  • Chen CL; Department of Nephrology, Landseed Hospital, Taoyuan, Taiwan.
  • Tsai JZ; Department of Electrical Engineering, National Central University, Jhongli, Taiwan. Electronic address: jztsai@ee.ncu.edu.tw.
Biosens Bioelectron ; 66: 244-50, 2015 Apr 15.
Article em En | MEDLINE | ID: mdl-25437359
In this study, we developed an interdigitated gold microelectrode-based impedance sensor to detect Escherichia coli (E. coli) in human urine samples for urinary tract infection (UTI) diagnosis. E. coli growth in human urine samples was successfully monitored during a 12-h culture, and the results showed that the maximum relative changes could be measured at 10Hz. An equivalent electrical circuit model was used for evaluating the variations in impedance characteristics of bacterial growth. The equivalent circuit analysis indicated that the change in impedance values at low frequencies was caused by double layer capacitance due to bacterial attachment and formation of biofilm on electrode surface in urine. A linear relationship between the impedance change and initial E. coli concentration was obtained with the coefficient of determination R(2)>0.90 at various growth times of 1, 3, 5, 7, 9 and 12h in urine. Thus our sensor is capable of detecting a wide range of E. coli concentration, 7×10(0) to 7×10(8) cells/ml, in urine samples with high sensitivity.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Urina / Técnicas Biossensoriais / Escherichia coli Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Revista: Biosens Bioelectron Assunto da revista: BIOTECNOLOGIA Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Taiwan País de publicação: Reino Unido

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Urina / Técnicas Biossensoriais / Escherichia coli Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Revista: Biosens Bioelectron Assunto da revista: BIOTECNOLOGIA Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Taiwan País de publicação: Reino Unido