CD71(high) population represents primitive erythroblasts derived from mouse embryonic stem cells.
Stem Cell Res
; 14(1): 30-8, 2015 Jan.
Article
em En
| MEDLINE
| ID: mdl-25485690
ABSTRACT
The CD71/Ter119 combination has been widely used to reflect dynamic maturation of erythrocytes in vivo. However, because CD71 is expressed on all proliferating cells, it is unclear whether it can be utilized as an erythrocyte-specific marker during differentiation of embryonic stem cells (ESCs). In this study, we revealed that a population expressing high level of CD71 (CD71(high)) during mouse ESC differentiation represented an in vitro counterpart of yolk sac-derived primitive erythroblasts (EryPs) isolated at 8.5days post coitum. In addition, these CD71(high) cells went through "maturational globin switching" and enucleated during terminal differentiation in vitro that were similar to the yolk sac-derived EryPs in vivo. We further demonstrated that the formation of CD71(high) population was regulated differentially by key factors including Scl, HoxB4, Eaf1, and Klf1. Taken together, our study provides a technical advance that allows efficient segregation of EryPs from differentiated ESCs in vitro for further understanding molecular regulation during primitive erythropoiesis.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Receptores da Transferrina
/
Antígenos CD
/
Eritroblastos
/
Células-Tronco Embrionárias
Limite:
Animals
Idioma:
En
Revista:
Stem Cell Res
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
China