Molecular characterization and expression of Lck in Nile tilapia (Oreochromis niloticus) in response to Streptococcus agalactiae stimulus.
Appl Biochem Biotechnol
; 175(5): 2376-89, 2015 Mar.
Article
em En
| MEDLINE
| ID: mdl-25492689
ABSTRACT
Lymphocyte-specific protein tyrosine kinase (Lck) plays a critical role in effective signal transductions that are fundamental to T cell differentiation, proliferation, and effector functions. In this paper, the Lck gene of Nile tilapia, Oreochromis niloticus (designated as On-Lck), was cloned and its expression pattern under the stimulation of Streptococcus agalactiae was investigated. Sequence analysis showed important structural characteristics required for T cell receptor (TCR) signal transduction were detected in the deduced amino acid sequence of On-Lck, and the deduced genomic structure of On-Lck was similar to the known Lck. In healthy Nile tilapia, the On-Lck transcripts were mainly detected in the thymus, spleen, head kidney, and gill. When immunized with inactivated S. agalactiae, the On-Lck mRNA expression was significantly upregulated in the thymus, spleen, and head kidney. Moreover, there was a clear time-dependent expression pattern of On-Lck after immunization, and the expression reached the highest level at 48 h in the spleen and thymus and at 72 h in the head kidney, respectively. This is the first report on the expression of Lck induced by intracellular bacteria vaccination in teleosts. These findings indicated that On-Lck may play an important role in the immune response to intracellular bacteria in Nile tilapia.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Infecções Estreptocócicas
/
Streptococcus agalactiae
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Proteína Tirosina Quinase p56(lck) Linfócito-Específica
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Proteínas de Peixes
/
Doenças dos Peixes
Limite:
Animals
Idioma:
En
Revista:
Appl Biochem Biotechnol
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
China