Altered AMP deaminase activity may extend postmortem glycolysis.
Meat Sci
; 102: 8-14, 2015 Apr.
Article
em En
| MEDLINE
| ID: mdl-25498483
ABSTRACT
Postmortem energy metabolism drives hydrogen accumulation in muscle and results in a fairly constant ultimate pH. Extended glycolysis results in adverse pork quality and may be possible with greater adenonucleotide availability postmortem. We hypothesized that slowing adenonucleotide removal by reducing AMP deaminase activity would extend glycolysis and lower the ultimate pH of muscle. Longissimus muscle samples were incorporated into an in vitro system that mimics postmortem glycolysis with or without pentostatin, an AMP deaminase inhibitor. Pentostatin lowered ultimate pH and increased lactate and glucose 6-phosphate with time. Based on these results and that AMPK γ3(R200Q) mutated pigs (RNâ») produce low ultimate pH pork, we hypothesized AMP deaminase abundance and activity would be lower in RNâ» muscle than wild-type. RNâ» muscle contained lower AMP deaminase abundance and activity. These data show that altering adenonucleotide availability postmortem can extend postmortem pH decline and suggest that AMP deaminase activity may, in part, contribute to the low ultimate pH observed in RNâ» pork.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Qualidade dos Alimentos
/
Músculo Esquelético
/
Armazenamento de Alimentos
/
Glicólise
/
AMP Desaminase
/
Carne
Limite:
Animals
País/Região como assunto:
America do norte
Idioma:
En
Revista:
Meat Sci
Assunto da revista:
CIENCIAS DA NUTRICAO
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
Estados Unidos