Whey protein plus bicarbonate supplement has little effects on structural atrophy and proteolysis marker immunopatterns in skeletal muscle disuse during 21 days of bed rest.
J Musculoskelet Neuronal Interact
; 14(4): 432-44, 2014 Dec.
Article
em En
| MEDLINE
| ID: mdl-25524969
ABSTRACT
OBJECTIVES:
To investigate the effect of whey protein plus potassium bicarbonate supplement on disused skeletal muscle structure and proteolysis after bed rest (BR).METHODS:
Soleus (SOL) and vastus lateralis (VL) biopsies were sampled from ten (n=10) healthy male subjects (aged 31±6 years) who did BR once with and once without protein supplement as a dietary countermeasure (cross-over study design). The structural changes (myofibre size and type distribution) were analysed by histological sections, and muscle protein breakdown indirectly via the proteolysis markers, calpain 1 and 3, calpastatin, MuRF1 and 2, both in muscle homogenates and by immunohistochemistry.RESULTS:
BR caused size-changes in myofiber cross-sectional area (FCSA, SOL, p=0,004; VL, p=0.03), and myofiber slow-to-fast type transition with increased hybrids (SOL, p=0.043; VL, p=0.037) however with campaign differences in SOL (p<0.033). No significant effect of BR and supplement was found by any of the key proteolysis markers.CONCLUSIONS:
Campaign differences in structural muscle adaptation may be an issue in cross-over design BR studies. The whey protein plus potassium bicarbonate supplement did not attenuate atrophy and fibre type transition during medium term bed rest. Alkaline whey protein supplements may however be beneficial as adjuncts to exercise countermeasures in disuse.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Repouso em Cama
/
Bicarbonatos
/
Atrofia Muscular
/
Compostos de Potássio
/
Proteólise
/
Proteínas do Leite
Tipo de estudo:
Clinical_trials
Limite:
Adult
/
Humans
/
Male
Idioma:
En
Revista:
J Musculoskelet Neuronal Interact
Assunto da revista:
FISIOLOGIA
/
NEUROLOGIA
/
ORTOPEDIA
Ano de publicação:
2014
Tipo de documento:
Article