Bioinformatic analysis of a PLP-dependent enzyme superfamily suitable for biocatalytic applications.
Biotechnol Adv
; 33(5): 566-604, 2015.
Article
em En
| MEDLINE
| ID: mdl-25575689
ABSTRACT
In this review we analyse structure/sequence-function relationships for the superfamily of PLP-dependent enzymes with special emphasis on class III transaminases. Amine transaminases are highly important for applications in biocatalysis in the synthesis of chiral amines. In addition, other enzyme activities such as racemases or decarboxylases are also discussed. The substrate scope and the ability to accept chemically different types of substrates are shown to be reflected in conserved patterns of amino acids around the active site. These findings are condensed in a sequence-function matrix, which facilitates annotation and identification of biocatalytically relevant enzymes and protein engineering thereof.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fosfato de Piridoxal
/
Biotecnologia
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Biologia Computacional
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Transaminases
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
Biotechnol Adv
Ano de publicação:
2015
Tipo de documento:
Article