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Merkel cell carcinoma: histopathologic and prognostic features according to the immunohistochemical expression of Merkel cell polyomavirus large T antigen correlated with viral load.
Leroux-Kozal, Valérie; Lévêque, Nicolas; Brodard, Véronique; Lesage, Candice; Dudez, Oriane; Makeieff, Marc; Kanagaratnam, Lukshe; Diebold, Marie-Danièle.
Afiliação
  • Leroux-Kozal V; Department of Pathology, Faculty of Medicine, Robert Debré University Hospital, 51092 Reims, France. Electronic address: lerouxvaleri@gmail.com.
  • Lévêque N; Clinical and Molecular Virology Unit, University Hospital, 51092 Reims, France; Faculty of Medicine, EA-4684 Cardiovir SFR-CAP Santé, 51092 Reims, France.
  • Brodard V; Clinical and Molecular Virology Unit, University Hospital, 51092 Reims, France.
  • Lesage C; Department of Dermatology, Robert Debré University Hospital, 51092 Reims, France.
  • Dudez O; Department of Pathology, Faculty of Medicine, Robert Debré University Hospital, 51092 Reims, France.
  • Makeieff M; Department of Otolaryngology-Head and Neck Surgery, Robert Debré University Hospital, 51092 Reims, France.
  • Kanagaratnam L; Department of Research and Innovation, Robert Debré University Hospital, 51092 Reims, France.
  • Diebold MD; Department of Pathology, Faculty of Medicine, Robert Debré University Hospital, 51092 Reims, France.
Hum Pathol ; 46(3): 443-53, 2015 Mar.
Article em En | MEDLINE | ID: mdl-25623078
Merkel cell carcinoma (MCC) is a neuroendocrine skin malignancy frequently associated with Merkel cell polyomavirus (MCPyV), which is suspected to be oncogenic. In a series of MCC patients, we compared clinical, histopathologic, and prognostic features according to the expression of viral large T antigen (LTA) correlated with viral load. We evaluated the LTA expression by immunohistochemistry using CM2B4 antibody and quantified viral load by real-time polymerase chain reaction. We analyzed formalin-fixed, paraffin-embedded (FFPE) tissue samples (n = 36) and corresponding fresh-frozen biopsies when available (n = 12), of the primary tumor and/or metastasis from 24 patients. MCPyV was detected in 88% and 58% of MCC patients by real-time polymerase chain reaction and immunohistochemistry, respectively. The relevance of viral load measurements was demonstrated by the strong consistency of viral load level between FFPE and corresponding frozen tissues as well as between primary tumor and metastases. From FFPE samples, 2 MCC subgroups were distinguished based on a viral load threshold defined by the positivity of CM2B4 immunostaining. In the LTA-negative subgroup with no or low viral load (nonsignificant), tumor cells showed more anisokaryosis (P = .01), and a solar elastosis around the tumor was more frequently observed (P = .03). LTA-positive MCCs with significant viral load had a lower proliferation index (P = .03) and a longer survival of corresponding patients (P = .008). Depending on MCPyV involvement, 2 MCC subgroups can be distinguished on histopathologic criteria, and the CM2B4 antibody is able to differentiate them reliably. Furthermore, the presence of a significant viral load in tumors is predictive of better prognosis.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias Cutâneas / Carcinoma de Célula de Merkel / Biomarcadores Tumorais / Antígenos Transformantes de Poliomavirus Tipo de estudo: Prognostic_studies Limite: Aged / Aged80 / Female / Humans / Male / Middle aged Idioma: En Revista: Hum Pathol Assunto da revista: PATOLOGIA Ano de publicação: 2015 Tipo de documento: Article País de publicação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias Cutâneas / Carcinoma de Célula de Merkel / Biomarcadores Tumorais / Antígenos Transformantes de Poliomavirus Tipo de estudo: Prognostic_studies Limite: Aged / Aged80 / Female / Humans / Male / Middle aged Idioma: En Revista: Hum Pathol Assunto da revista: PATOLOGIA Ano de publicação: 2015 Tipo de documento: Article País de publicação: Estados Unidos