Microdroplet digital PCR: detection and quantitation of biomarkers in archived tissue and serial plasma samples in patients with lung cancer.
J Thorac Oncol
; 10(1): 212-7, 2015 Jan.
Article
em En
| MEDLINE
| ID: mdl-25654729
ABSTRACT
INTRODUCTION:
There is much interest in the use of noninvasive biomarkers in the management of lung cancer, particularly with respect to early diagnosis and monitoring the response to intervention. Cell-free tumor DNA in patients with cancer has been shown to hold potential as a noninvasive biomarker, in which the response to treatment may be evaluated using a blood test only. Multiple technologies have been suggested as being appropriate to measure cell-free tumor DNA. Microdroplet digital polymerase chain reaction (mdPCR) has a number of attributes that suggest it may be a useful tool for detecting clinically relevant genetic events. It offers precise and accurate quantitation of mutant alleles, including rare variants.METHODS:
We evaluate the performance of mdPCR in the analysis of DNA extracted from reference standards, tumor biopsies, and patient plasma.RESULTS:
The potential of mdPCR to detect clinically relevant mutations is demonstrated, in both formalin-fixed paraffin-embedded material and plasma. Furthermore, we show that mdPCR can be used to track changes in peripheral blood biomarkers in response to treatment and to detect the emergence of drug-resistant clones.CONCLUSIONS:
MdPCR has potential as a tool to detect and quantify tumor-derived mutational events in cell-free DNA from patients with lung cancer.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Análise Mutacional de DNA
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Adenocarcinoma
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Biomarcadores Tumorais
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Reação em Cadeia da Polimerase
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Neoplasias Pulmonares
Tipo de estudo:
Diagnostic_studies
/
Guideline
/
Screening_studies
Limite:
Female
/
Humans
/
Male
Idioma:
En
Revista:
J Thorac Oncol
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
Reino Unido