Porcine aminopeptidase N mediated polarized infection by porcine epidemic diarrhea virus in target cells.
Virology
; 478: 1-8, 2015 Apr.
Article
em En
| MEDLINE
| ID: mdl-25681796
ABSTRACT
Infection of polarized intestinal epithelial cells by porcine epidemic diarrhea virus (PEDV) was characterized. Indirect immunofluorescence assay, real-time PCR, and transmission electron microscopy confirmed PEDV can be successfully propagated in immortalized swine small intestine epithelial cells (IECs). Infection involved porcine aminpeptidase N (pAPN), a reported cellular receptor for PEDV, transient expression of pAPN and siRNA targeted pAPN increased and decreased the infectivity of PEDV in IECs, respectively. Subsequently, polarized entry into and release from both Vero E6 and IECs was analyzed. PEDV entry into polarized cells and pAPN grown on membrane inserts occurs via apical membrane. The progeny virus released into the medium was also quantified which demonstrated that PEDV is preferentially released from the apical membrane. Collectively, our data demonstrate that pAPN, the cellular receptor for PEDV, mediates polarized PEDV infection. These results imply the possibility that PEDV infection may proceed by lateral spread of virus in intestinal epithelial cells.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Receptores Virais
/
Antígenos CD13
/
Vírus da Diarreia Epidêmica Suína
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Internalização do Vírus
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Interações Hospedeiro-Patógeno
Limite:
Animals
Idioma:
En
Revista:
Virology
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
China