Construction of a artificial glutathione peroxidase with temperature-dependent activity based on a supramolecular graft copolymer.

A supramolecular artificial glutathione peroxidase (PNIPAM-CD-g-Te) was prepared based on a supramolecular graft copolymer. PNIPAM-CD-g-Te was constructed by supramolecular host-guest self-assembly. Significantly, PNIPAM-CD-g-Te displayed noticeable temperature-dependent catalytic activity and typical saturation kinetics behavior. It was also proved that the change in the self-assembled structure of PNIPAM-CD-g-Te during the temperature-dependent process played a significant role in the temperature-dependent catalytic behavior. The construction of PNIPAM-CD-g-Te based on supramolecular graft copolymer endows artificial GPx with temperature-dependent catalytic ability, enriched catalytic centers, and homogeneously distributed catalytic centers. This work bodes well for the development of other biologically related host-guest supramolecular biomaterials.